Soluble N-Acetylgalactosamine-Modified Antigens Enhance Hepatocyte-Dependent Antigen Cross-Presentation and Result in Antigen-Specific CD8
ATP Binding Cassette Transporter, Subfamily B, Member 2
/ genetics
Acetylgalactosamine
/ immunology
Adoptive Transfer
/ methods
Animals
Antigen Presentation
/ immunology
Antigens
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Cells, Cultured
Cross-Priming
/ immunology
Hepatocytes
/ cytology
Immune Tolerance
/ immunology
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Ovalbumin
/ immunology
Skin Transplantation
/ methods
Solubility
Vesicular Transport Proteins
/ immunology
CD8 T cell
antigen modifications
cross-presentation/priming
hepatocytes
tolerance
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
23
04
2020
accepted:
10
02
2021
entrez:
22
3
2021
pubmed:
23
3
2021
medline:
29
6
2021
Statut:
epublish
Résumé
Hepatocytes compose up to 80% of the total liver and have been indicated as important players in the induction of immunologic tolerance in this organ. We show that hepatocytes possess the molecular machinery required for the cross-presentation of extracellular antigens. Using a derivative of the model antigen ovalbumin (OVA) covalently modified with a polymer containing multiple N-acetylgalactosamine residues (pGal-OVA) that enhance extracellular antigen uptake by mimicking the glycome of apoptotic debris, we show efficient hepatocyte-dependent induction of cross-tolerance of both adoptively transferred OT-I cells and endogenous OVA-specific CD8
Identifiants
pubmed: 33746941
doi: 10.3389/fimmu.2021.555095
pmc: PMC7965950
doi:
Substances chimiques
ATP Binding Cassette Transporter, Subfamily B, Member 2
0
Antigens
0
Tap1 protein, mouse
0
Vesicular Transport Proteins
0
early endosome antigen 1
0
Ovalbumin
9006-59-1
Acetylgalactosamine
KM15WK8O5T
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
555095Informations de copyright
Copyright © 2021 Damo, Wilson, Watkins and Hubbell.
Déclaration de conflit d'intérêts
The EPFL has filed for patent protection on the pGal-antigen delivery platform, and MD, DW, and JH are named as inventors on those patents. Anokion SA and Kanyos Bio, Inc., have licensed those patents, and JH and DW participate in equity in those companies. The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Sci Transl Med. 2015 May 27;7(289):289ra81
pubmed: 26019217
Hepatology. 2011 Oct;54(4):1379-87
pubmed: 21721032
Blood. 2007 Dec 15;110(13):4144-52
pubmed: 17726165
Science. 2007 Apr 27;316(5824):612-6
pubmed: 17463291
J Clin Exp Hepatol. 2011 Sep;1(2):109-14
pubmed: 25755322
PLoS One. 2014 Aug 19;9(8):e103755
pubmed: 25137039
Annu Rev Immunol. 2009;27:147-63
pubmed: 19302037
Auto Immun Highlights. 2012 Oct 30;3(3):119-25
pubmed: 26000135
J Autoimmun. 2010 Feb;34(1):15-22
pubmed: 19744829
J Immunol Res. 2015;2015:450695
pubmed: 26839900
Autoimmunity. 2007 Jun;40(4):249-53
pubmed: 17516205
Trends Immunol. 2005 Feb;26(2):104-10
pubmed: 15668126
J Immunol. 2011 Sep 1;187(5):2405-17
pubmed: 21821796
Hepatology. 2009 Sep;50(3):909-19
pubmed: 19610048
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1217-21
pubmed: 1899924
Eur J Immunol. 2008 Apr;38(4):957-67
pubmed: 18383043
FEBS Lett. 1992 Jan 20;296(2):174-8
pubmed: 1370803
Cell Rep. 2013 Mar 28;3(3):779-95
pubmed: 23499443
Glycobiology. 2012 Jun;22(6):788-805
pubmed: 22345628
J Clin Invest. 2004 Sep;114(5):701-12
pubmed: 15343389
J Immunol Methods. 1999 Feb 1;223(1):77-92
pubmed: 10037236
J Immunol. 2007 Jun 1;178(11):6849-60
pubmed: 17513733
Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16735-40
pubmed: 21933957
Eur J Immunol. 2005 Oct;35(10):2970-81
pubmed: 16163670
Nat Rev Immunol. 2003 Jan;3(1):51-62
pubmed: 12511875
J Exp Med. 2000 Dec 18;192(12):1685-96
pubmed: 11120766
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):E2540-9
pubmed: 24927525
Hepatology. 2008 Jan;47(1):296-305
pubmed: 17975811
J Exp Med. 2000 Feb 7;191(3):411-6
pubmed: 10662786
J Cell Sci. 2010 Oct 1;123(Pt 19):3347-56
pubmed: 20826457
Bioessays. 1994 Jul;16(7):519-24
pubmed: 7945281
Int Immunol. 1999 Aug;11(8):1225-38
pubmed: 10421780
Cell Rep. 2012 Mar 29;1(3):191-9
pubmed: 22832193
Nat Biomed Eng. 2019 Oct;3(10):817-829
pubmed: 31358881
Blood. 2009 Dec 10;114(25):5152-61
pubmed: 19794140
Nature. 2003 Sep 25;425(6956):397-402
pubmed: 14508489
Immunol Rev. 2006 Oct;213:101-18
pubmed: 16972899
J Immunol. 2007 Mar 1;178(5):2679-87
pubmed: 17312109
Proteomics. 2011 Sep;11(17):3556-64
pubmed: 21751380
Clin Immunol. 2007 Feb;122(2):125-34
pubmed: 17029966
Microsc Res Tech. 2002 Jun 15;57(6):530-40
pubmed: 12112436
Nat Immunol. 2008 May;9(5):558-66
pubmed: 18376402
Pathobiology. 1999;67(5-6):302-5
pubmed: 10725808
J Immunol. 2014 Jun 1;192(11):5002-11
pubmed: 24795456
J Hepatol. 2014 Sep;61(3):594-9
pubmed: 24798620
JCI Insight. 2018 Aug 9;3(15):
pubmed: 30089715