Feasibility and Performance of Elastin Trichrome as a Primary Stain in Colorectal Cancer Resection Specimens: Results of an Interobserver Variability Study.
Azo Compounds
Biomarkers, Tumor
/ analysis
Colorectal Neoplasms
/ chemistry
Coloring Agents
Elastin
/ analysis
Eosine Yellowish-(YS)
Feasibility Studies
Humans
Methyl Green
Neoplasm Invasiveness
Observer Variation
Predictive Value of Tests
Reproducibility of Results
Staining and Labeling
Veins
/ chemistry
Journal
The American journal of surgical pathology
ISSN: 1532-0979
Titre abrégé: Am J Surg Pathol
Pays: United States
ID NLM: 7707904
Informations de publication
Date de publication:
01 10 2021
01 10 2021
Historique:
pubmed:
24
3
2021
medline:
5
10
2021
entrez:
23
3
2021
Statut:
ppublish
Résumé
Venous invasion (VI) is a powerful prognostic factor in colorectal cancer (CRC) that is widely underreported. The ability of elastin stains to improve VI detection is now recognized in several international CRC pathology protocols. However, concerns related to the cost and time required to perform and evaluate these stains in addition to routine hematoxylin and eosin (H&E) stains remains a barrier to their wider use. We therefore sought to determine whether an elastin trichrome (ET) stain could be used as a "stand-alone" stain in CRC resections, by comparing the sensitivity, accuracy, and reproducibility of detection of CAP-mandated prognostic factors using ET and H&E stains. Representative H&E- and ET-stained slides from 50 CRC resections, including a representative mix of stages and prognostic factors, were used to generate 2 study sets. Each case was represented by H&E slides in 1 study set and by corresponding ET slides from the same blocks in the other study set. Ten observers (3 academic gastrointestinal [GI] pathologists, 4 community pathologists, 3 fellows) evaluated each study set for CAP-mandated prognostic factors. ET outperformed H&E in the assessment of VI with respect to detection rates (50% vs. 28.6%; P<0.0001), accuracy (82% vs. 59%, P<0.0001), and reproducibility (k=0.554 vs. 0.394). No significant differences between ET and H&E were observed for other features evaluated. In a poststudy survey, most observers considered the ease and speed of assessment at least equivalent for ET and H&E for most prognostic factors, and felt that ET would be feasible as a stand-alone stain in practice. If validated by others, our findings support the use of ET, rather than H&E, as the primary stain for the evaluation of CRC resections.
Identifiants
pubmed: 33756495
doi: 10.1097/PAS.0000000000001707
pii: 00000478-202110000-00014
doi:
Substances chimiques
Azo Compounds
0
Biomarkers, Tumor
0
Coloring Agents
0
ELN protein, human
0
trichrome stain
0
Methyl Green
82-94-0
Elastin
9007-58-3
Eosine Yellowish-(YS)
TDQ283MPCW
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1419-1427Informations de copyright
Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflicts of Interest and Source of Funding: The authors have disclosed that they have no significant relationships with, or financial interest in, any commercial companies pertaining to this article.
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