Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies.
DEER
EPR
In-cell EPR
Nitroxide radicals
SDSL
Journal
Biochimica et biophysica acta. Proteins and proteomics
ISSN: 1878-1454
Titre abrégé: Biochim Biophys Acta Proteins Proteom
Pays: Netherlands
ID NLM: 101731734
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
31
10
2020
revised:
17
03
2021
accepted:
18
03
2021
pubmed:
25
3
2021
medline:
21
9
2021
entrez:
24
3
2021
Statut:
ppublish
Résumé
Site-Directed Spin Labelling (SDSL) technique is based on the attachment of a paramagnetic label onto a specific position of a protein (or other bio-molecules) and the subsequent study by Electron Paramagnetic Resonance (EPR) spectroscopy. In particular, continuous-wave EPR (cw-EPR) spectra can detect the local conformational dynamics for proteins under various conditions. Moreover, pulse-EPR experiments on doubly spin-labelled proteins allow measuring distances between spin centres in the 1.5-8 nm range, providing information about structures and functions. This review focuses on SDSL-EPR spectroscopy as a structural biology tool to investigate proteins using nitroxide labels. The versatility of this spectroscopic approach for protein structural characterization has been demonstrated through the choice of recent studies. The main aim is to provide a general overview of the technique, particularly for non-experts, to spread the applicability of this technique in various fields of structural biology.
Identifiants
pubmed: 33757896
pii: S1570-9639(21)00059-5
doi: 10.1016/j.bbapap.2021.140653
pii:
doi:
Substances chimiques
Nitrogen Oxides
0
Proteins
0
Spin Labels
0
nitroxyl
GFQ4MMS07W
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
140653Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.