Cancer-associated cells release citrate to support tumour metastatic progression.


Journal

Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869

Informations de publication

Date de publication:
06 2021
Historique:
received: 04 09 2020
revised: 09 03 2021
accepted: 10 03 2021
entrez: 24 3 2021
pubmed: 25 3 2021
medline: 13 10 2021
Statut: epublish

Résumé

Citrate is important for lipid synthesis and epigenetic regulation in addition to ATP production. We have previously reported that cancer cells import extracellular citrate via the pmCiC transporter to support their metabolism. Here, we show for the first time that citrate is supplied to cancer by cancer-associated stroma (CAS) and also that citrate synthesis and release is one of the latter's major metabolic tasks. Citrate release from CAS is controlled by cancer cells through cross-cellular communication. The availability of citrate from CAS regulated the cytokine profile, metabolism and features of cellular invasion. Moreover, citrate released by CAS is involved in inducing cancer progression especially enhancing invasiveness and organ colonisation. In line with the in vitro observations, we show that depriving cancer cells of citrate using gluconate, a specific inhibitor of pmCiC, significantly reduced the growth and metastatic spread of human pancreatic cancer cells in vivo and muted stromal activation and angiogenesis. We conclude that citrate is supplied to tumour cells by CAS and citrate uptake plays a significant role in cancer metastatic progression.

Identifiants

pubmed: 33758075
pii: 4/6/e202000903
doi: 10.26508/lsa.202000903
pmc: PMC7994318
pii:
doi:

Substances chimiques

Citric Acid 2968PHW8QP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2021 Drexler et al.

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Auteurs

Konstantin Drexler (K)

Department of Dermatology, University Medical Centre, Regensburg, Germany.

Katharina M Schmidt (KM)

Department of Surgery, University Medical Center, Regensburg, Germany.

Katrin Jordan (K)

Department of Surgery, University Medical Center, Regensburg, Germany.

Marianne Federlin (M)

Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.

Vladimir M Milenkovic (VM)

Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany.

Gerhard Liebisch (G)

Institute of Clinical Chemistry and Laboratory Medicine, Regensburg University Hospital, Regensburg, Germany.

Anna Artati (A)

Research Unit Molecular Endocrinology and Metabolism, Helmholtz Zentrum München, German Research Centre for Environmental Health, Neuherberg, Germany.

Christian Schmidl (C)

Regensburg Center for Interventional Immunology, Regensburg, Germany.

Gregor Madej (G)

Department of Structural Biology, Institute of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany.

Janina Tokarz (J)

Research Unit Molecular Endocrinology and Metabolism, Helmholtz Zentrum München, German Research Centre for Environmental Health, Neuherberg, Germany.

Alexander Cecil (A)

Research Unit Molecular Endocrinology and Metabolism, Helmholtz Zentrum München, German Research Centre for Environmental Health, Neuherberg, Germany.

Wolfgang Jagla (W)

Institute of Pathology, Kaufbeuren-Ravensburg, Kaufbeuren, Germany.

Silke Haerteis (S)

Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany.

Thiha Aung (T)

Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany.
Center of Plastic, Aesthetic, Hand and Reconstructive Surgery, University of Regensburg, Regensburg, Germany.

Christine Wagner (C)

Department of Surgery, University Medical Center, Regensburg, Germany.

Maria Kolodziejczyk (M)

Department of Surgery, University Medical Center, Regensburg, Germany.

Stefanie Heinke (S)

Department of Surgery, University Medical Center, Regensburg, Germany.

Evan H Stanton (EH)

Department of Surgery, University Medical Center, Regensburg, Germany.

Barbara Schwertner (B)

Department of Dermatology, University Medical Centre, Regensburg, Germany.

Dania Riegel (D)

Regensburg Center for Interventional Immunology, Regensburg, Germany.

Christian H Wetzel (CH)

Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany.

Wolfgang Buchalla (W)

Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.

Martin Proescholdt (M)

Department of Neurosurgery, University Hospital Regensburg, Regensburg, Germany.

Christoph A Klein (CA)

Experimental Medicine and Therapy Research, University of Regensburg, Regensburg, Germany.

Mark Berneburg (M)

Department of Dermatology, University Medical Centre, Regensburg, Germany.

Hans J Schlitt (HJ)

Department of Surgery, University Medical Center, Regensburg, Germany.

Thomas Brabletz (T)

Department of Experimental Medicine 1, Friedrich-Alexander-University Erlangen, Erlangen, Germany.

Christine Ziegler (C)

Department of Structural Biology, Institute of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany.

Eric K Parkinson (EK)

Centre for Immunobiology and Regenerative Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, London, UK.

Andreas Gaumann (A)

Institute of Pathology, Kaufbeuren-Ravensburg, Kaufbeuren, Germany.

Edward K Geissler (EK)

Department of Surgery, University Medical Center, Regensburg, Germany.

Jerzy Adamski (J)

Research Unit Molecular Endocrinology and Metabolism, Helmholtz Zentrum München, German Research Centre for Environmental Health, Neuherberg, Germany.
Lehrstuhl für Experimentelle Genetik, Technische Universität München, Munich, Germany.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Sebastian Haferkamp (S)

Department of Dermatology, University Medical Centre, Regensburg, Germany sebastian.haferkamp@ukr.de.

Maria E Mycielska (ME)

Department of Surgery, University Medical Center, Regensburg, Germany maria.mycielska@ukr.de.

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Classifications MeSH