Evaluation of the efficacy of enrofloxacin in rainbow trout (Oncorhynchus mykiss) following experimental challenge with Yersinia ruckeri.


Journal

The Veterinary record
ISSN: 2042-7670
Titre abrégé: Vet Rec
Pays: England
ID NLM: 0031164

Informations de publication

Date de publication:
Jun 2021
Historique:
revised: 05 12 2020
received: 23 07 2020
accepted: 08 02 2021
pubmed: 25 3 2021
medline: 3 8 2021
entrez: 24 3 2021
Statut: ppublish

Résumé

Use of enrofloxacin in trout farms is reported, especially for the treatment of yersiniosis, albeit various dosing regimens have been used. Therefore, optimal doses should be investigated. Five groups of 15 fish were challenged with Y. ruckeri. Two days later, three groups received feed containing enrofloxacin (ENR) at 1, 2.5 and 5 mg/kg fish respectively, during 7 days; one group received a single intraperitoneal injection of ENR at 10 mg/kg; and one group was left untreated. On day 15, surviving fish were euthanized. All fish survived in the group treated by injection, compared to 53%, 60% and 40% of the fish treated with 1, 2.5 and 5 mg/kg oral ENR, respectively, and 53% in the infected untreated group. A single intraperitoneal injection of ENR at 10 mg/kg seems more relevant than repeated oral administrations. The ENR oral doses used in trout farms should be revised.

Sections du résumé

BACKGROUND BACKGROUND
Use of enrofloxacin in trout farms is reported, especially for the treatment of yersiniosis, albeit various dosing regimens have been used. Therefore, optimal doses should be investigated.
METHODS METHODS
Five groups of 15 fish were challenged with Y. ruckeri. Two days later, three groups received feed containing enrofloxacin (ENR) at 1, 2.5 and 5 mg/kg fish respectively, during 7 days; one group received a single intraperitoneal injection of ENR at 10 mg/kg; and one group was left untreated. On day 15, surviving fish were euthanized.
RESULTS RESULTS
All fish survived in the group treated by injection, compared to 53%, 60% and 40% of the fish treated with 1, 2.5 and 5 mg/kg oral ENR, respectively, and 53% in the infected untreated group.
CONCLUSION CONCLUSIONS
A single intraperitoneal injection of ENR at 10 mg/kg seems more relevant than repeated oral administrations. The ENR oral doses used in trout farms should be revised.

Identifiants

pubmed: 33759199
doi: 10.1002/vetr.200
doi:

Substances chimiques

Enrofloxacin 3DX3XEK1BN

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e200

Subventions

Organisme : France-AGRIMER
Organisme : CIPA, FEP and the regions of Aquitaine
Organisme : Brittany and Pays de la Loire
ID : CIM 6008-1420

Informations de copyright

© 2021 British Veterinary Association.

Références

FAO. The State of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all. Rome: FAO; 2016. ISBN 978-92-5-109185-2.
Reimschuessel R, Miller RA, Gieseker CM. Antimicrobial drug use in aquaculture. In: Steeve Giguère, John F. Prescott, Patricia M. Dowling, editors. Antimicrobial therapy in veterinary medicine. 5th ed. Ames: Wiley Blackwell; 2013. p. 645-61.
Calvez S, Gantelet H, Blanc G, Douet D-G, Daniel P. Yersinia ruckeri Biotypes 1 and 2 in France: presence and antibiotic susceptibility. Dis Aquat Org. 2014;109(2):117-26.
Huang Y, Michael GB, Becker R, Kaspar H, Mankertz J, Schwarz S, et al. Pheno- and genotypic analysis of antimicrobial resistance properties of Yersinia ruckeri from fish. Vet Microbiol. 2014;171(3-4):406-12.
Rodgers CJ, Furones MD. Antimicrobial agents in aquaculture: practice, nedds and issues. In: Rogers C., Basurco B. editors. The use of veterinary drugs and vaccines in Mediterranean aquaculture. Zaragoza: CIHEAM; 2009. p. 41-59.
Gibello A, Blanco MM, Moreno MA, Cutuli MT, Domenech A, Domínguez L, et al. Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout. Appl Environ Microbiol. 1999;65(1): 346-50.
Haig SJ, Davies RL, Welch TJ, Reese RA, Verner-Jeffreys DW. Comparative susceptibility of Atlantic salmon and rainbow trout to Yersinia ruckeri: relationship to O antigen serotype and resistance to serum killing. Vet Microbiol. 2011;147(1-2):155-61.
Str⊘m HK, Ohtani M, Nowak B, Boutrup TS, Jones B, Raida MK, et al. Experimental infection by Yersinia ruckeri O1 biotype 2 induces brain lesions and neurological signs in rainbow trout (Oncorhynchus mykiss). J Fish Dis. 2018;41(3):529-37.
Bowser PR, Wooster GA, St Leger J, Babish JG. Pharmacokinetics of enrofloxacin in fingerling rainbow trout (Oncorhynchus mykiss). J Vet Pharmacol Ther. 1992;15(1):62-71.
Urzúa N, Messina MJ, Prieto G, Lüders C, Errecalde C. Pharmacokinetics and tissue disposition of enrofloxacin in rainbow trout after different routes of administration. Xenobiotica. 2020;50(10):1236-41.
Coyne R, Samuelsen O, Kongshaug H, Andersen K, Dalsgaard I, Smith P, et al. A comparison of oxolinic acid concentrations in farmed and laboratory held rainbow trout (Oncorhynchus mykiss) following oral therapy. Aquaculture. 2004;239(1):1-13.

Auteurs

Antoine Rostang (A)

INRAE, Oniris, BIOEPAR, Nantes, France.

Carole Peroz (C)

INRAE, Oniris, BIOEPAR, Nantes, France.

Catherine Fournel (C)

INRAE, Oniris, BIOEPAR, Nantes, France.

Chantal Thorin (C)

NP3, Oniris, Nantes, France.

Ségolène Calvez (S)

INRAE, Oniris, BIOEPAR, Nantes, France.

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