Plk4 triggers autonomous de novo centriole biogenesis and maturation.
Animals
Cell Cycle
/ physiology
Cell Cycle Proteins
/ metabolism
Cell Division
/ physiology
Cells, Cultured
Centrioles
/ metabolism
Centrosome
/ metabolism
Drosophila Proteins
/ metabolism
Drosophila melanogaster
/ metabolism
Female
Male
Protein Serine-Threonine Kinases
/ metabolism
Tubulin
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 05 2021
03 05 2021
Historique:
received:
17
08
2020
revised:
14
12
2020
accepted:
18
02
2021
entrez:
24
3
2021
pubmed:
25
3
2021
medline:
29
9
2021
Statut:
ppublish
Résumé
Centrioles form centrosomes and cilia. In most proliferating cells, centrioles assemble through canonical duplication, which is spatially, temporally, and numerically regulated by the cell cycle and the presence of mature centrioles. However, in certain cell types, centrioles assemble de novo, yet by poorly understood mechanisms. Herein, we established a controlled system to investigate de novo centriole biogenesis, using Drosophila melanogaster egg explants overexpressing Polo-like kinase 4 (Plk4), a trigger for centriole biogenesis. We show that at a high Plk4 concentration, centrioles form de novo, mature, and duplicate, independently of cell cycle progression and of the presence of other centrioles. Plk4 concentration determines the temporal onset of centriole assembly. Moreover, our results suggest that distinct biochemical kinetics regulate de novo and canonical biogenesis. Finally, we investigated which other factors modulate de novo centriole assembly and found that proteins of the pericentriolar material (PCM), and in particular γ-tubulin, promote biogenesis, likely by locally concentrating critical components.
Identifiants
pubmed: 33760919
pii: 211915
doi: 10.1083/jcb.202008090
pmc: PMC7995200
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
Drosophila Proteins
0
Tubulin
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Informations de copyright
© 2021 Nabais et al.
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