Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe.
Animals
Aorta
/ pathology
Apolipoproteins E
/ deficiency
Atherosclerosis
/ pathology
Cell Death
Fluorescence
Fluorescent Dyes
/ chemistry
Human Umbilical Vein Endothelial Cells
/ metabolism
Humans
Hydrogen Peroxide
/ chemistry
Intravital Microscopy
Iron
/ chemistry
Liposomes
Macrophages
/ metabolism
Mice
Mice, Inbred C57BL
Optical Imaging
Organ Specificity
Oxidation-Reduction
Oxidative Stress
Reactive Oxygen Species
/ metabolism
Spectroscopy, Near-Infrared
THP-1 Cells
Tyrosine
/ analogs & derivatives
Up-Regulation
Vascular Cell Adhesion Molecule-1
/ metabolism
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
11
2020
revised:
28
01
2021
accepted:
05
02
2021
entrez:
25
3
2021
pubmed:
26
3
2021
medline:
20
5
2021
Statut:
epublish
Résumé
Excessive production of reactive oxygen species (ROS) and the ensuing oxidative stress are instrumental in all phases of atherosclerosis. Despite the major achievements in understanding the regulatory pathways and molecular sources of ROS in the vasculature, the specific detection and quantification of ROS in experimental models of disease remain a challenge. We aimed to develop a reliable and straightforward imaging procedure to interrogate the ROS overproduction in the vasculature and in various organs/tissues in atherosclerosis. To this purpose, the cell-impermeant ROS Brite™ 700 (RB700) probe that produces bright near-infrared fluorescence upon ROS oxidation was encapsulated into VCAM-1-targeted, sterically stabilized liposomes (VLp). Cultured human endothelial cells (EC) and macrophages (Mac) were used for
Identifiants
pubmed: 33763173
doi: 10.1155/2021/6685612
pmc: PMC7963910
doi:
Substances chimiques
Apolipoproteins E
0
Fenton's reagent
0
Fluorescent Dyes
0
Liposomes
0
Reactive Oxygen Species
0
Vascular Cell Adhesion Molecule-1
0
3-nitrotyrosine
3604-79-3
Tyrosine
42HK56048U
Hydrogen Peroxide
BBX060AN9V
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6685612Informations de copyright
Copyright © 2021 Simona-Adriana Manea et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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