Understanding DNA organization, damage, and repair with super-resolution fluorescence microscopy.
DNA damage
DNA structure
chromatin
genomic integrity
super resolution microscopy
Journal
Methods and applications in fluorescence
ISSN: 2050-6120
Titre abrégé: Methods Appl Fluoresc
Pays: England
ID NLM: 101608648
Informations de publication
Date de publication:
05 May 2021
05 May 2021
Historique:
received:
13
12
2020
accepted:
25
03
2021
pubmed:
26
3
2021
medline:
21
10
2021
entrez:
25
3
2021
Statut:
epublish
Résumé
Super-resolution microscopy (SRM) comprises a suite of techniques well-suited to probing the nanoscale landscape of genomic function and dysfunction. Offering the specificity and sensitivity that has made conventional fluorescence microscopy a cornerstone technique of biological research, SRM allows for spatial resolutions as good as 10 nanometers. Moreover, single molecule localization microscopies (SMLMs) enable examination of individual molecular targets and nanofoci allowing for the characterization of subpopulations within a single cell. This review describes how key advances in both SRM techniques and sample preparation have enabled unprecedented insights into DNA structure and function, and highlights many of these new discoveries. Ongoing development and application of these novel, highly interdisciplinary SRM assays will continue to expand the toolbox available for research into the nanoscale genomic landscape.
Identifiants
pubmed: 33765677
doi: 10.1088/2050-6120/abf239
doi:
Substances chimiques
Chromatin
0
Fluorescent Dyes
0
Histones
0
Molecular Probes
0
DNA
9007-49-2
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Creative Commons Attribution license.