Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents.
Adolescent
Asthma
/ complications
Biomarkers
/ blood
Child
Cross-Sectional Studies
Female
Gene Expression
Humans
Interleukin-17
/ blood
Leukocytes
/ immunology
Male
Nuclear Receptor Subfamily 1, Group F, Member 3
/ genetics
Obesity
/ complications
Phenotype
RNA, Messenger
/ blood
Th17 Cells
/ immunology
Tumor Necrosis Factor-alpha
/ blood
Asthma
IL-17A
Non-allergic asthma
Obesity
RORC
Tumour necrosis factor-alpha
Journal
Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
05
11
2020
revised:
10
03
2021
accepted:
21
03
2021
pubmed:
28
3
2021
medline:
18
9
2021
entrez:
27
3
2021
Statut:
ppublish
Résumé
Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.
Identifiants
pubmed: 33771687
pii: S1521-6616(21)00052-8
doi: 10.1016/j.clim.2021.108715
pii:
doi:
Substances chimiques
Biomarkers
0
IL17A protein, human
0
Interleukin-17
0
Nuclear Receptor Subfamily 1, Group F, Member 3
0
RNA, Messenger
0
RORC protein, human
0
TNF protein, human
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108715Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.