Genetic diversity of Syphacia Seurat, 1916 (Nematoda: Oxyuridae) across the hybrid zone of their rodent hosts in Russia.
Apodemus
Microtus spp.
Phylogeny
Pinworms
Sylvaemus
Taxonomy
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
28
05
2020
accepted:
21
03
2021
pubmed:
28
3
2021
medline:
22
6
2021
entrez:
27
3
2021
Statut:
ppublish
Résumé
The genetic diversity of Syphacia nematodes (intestinal parasites of rodents) was studied in the hybrid zone of two sister species of common voles, Microtus arvalis and Microtus obscurus, in the Oka River valley, east of Moscow. Syphacia nematodes of other rodent species (Microtus rossiaemeridionalis, Alexandromys oeconomus, Sylvaemus uralensis, and Apodemus agrarius) that inhabit the area were also studied. Phylogenetic trees for the studied nematodes were inferred from the analysis of nuclear ITS1+5.8S+ITS2, LSU rDNA, and mitochondrial CO1 gene partial sequences. Syphacia nematodes of the studied area form three well-defined clades in the phylogenetic tree of this genus. Morphological analysis revealed similarities between the obtained sequences with those of known Syphacia species from the GenBank database, which enabled identifying these three clades up to the species level: S. montana, S. agraria, and S. frederici. Russian haplotypes of Syphacia are different from West European and East Asian haplotypes with pronounced genetic distances. A high level of specificity was reported for two of these three species (S. frederici, only in Sylvaemus uralensis; S. agraria, only in Apodemus agrarius). S. montana was found in different species of voles. Remarkably, S. montana specimens from M. arvalis and M. obscurus were genetically uniform, while S. montana, specimens from hybrids between these two species formed a separate clade distant from those originating from non-hybridised hosts.
Identifiants
pubmed: 33772348
doi: 10.1007/s00436-021-07134-7
pii: 10.1007/s00436-021-07134-7
doi:
Substances chimiques
DNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2017-2030Subventions
Organisme : Russian Foundation for Basic Research
ID : 19-74-20147
Références
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25:3389–3402. https://doi.org/10.1093/nar/25.17.3389
doi: 10.1093/nar/25.17.3389
pubmed: 9254694
Baird SJE, Ribas A, Macholán M, Albrecht T, Piálek J, Goüy de Bellocq J (2012) Where are the wormy mice? A reexamination of hybrid parasitism in the European house mouse hybrid zone. Evolution: International Journal of Organic Evolution 66:2757–2772. https://doi.org/10.1111/j.1558-5646.2012.01633.x
doi: 10.1111/j.1558-5646.2012.01633.x
Brant SV, Orti G (2003) Evidence for gene flow in parasitic nematodes between two host species of shrews. Mol Ecol 12:2853–2859. https://doi.org/10.1046/j.1365-294x.2003.01927.x
doi: 10.1046/j.1365-294x.2003.01927.x
pubmed: 12969487
Bulatova NS, Golenishchev FN, Koval’skaya YM, Emelyanova LG, Bystrakova NV, Pavlova SV, Nadzhafova RS, Lavrenchenko LA (2010a) Cytogenetic study of the parapatric contact zone between two 46-chromosomal forms of the common vole in European Russia. Russian Journal of Genetics 46:443–448. https://doi.org/10.1134/S1022795410040095
doi: 10.1134/S1022795410040095
Bulatova NS, Potapov SG, Lavrenchenko LA (2010b) Genomic versus chromosomal polytypy in studies of mitochondrial and nuclear DNA markers in the Microtus arvalis group. Russian Journal of Genetics 46:586–594. https://doi.org/10.1134/S1022795410050121
doi: 10.1134/S1022795410050121
Chenna R, Sugawara H, Koike T, Lope R, Gibson TJ, Higgins DG, Thompson JD (2003) Multiple sequence alignment with the Clustal series of programs. Nucl Acids Res 31:3497–3500. https://doi.org/10.1093/nar/gkg500
doi: 10.1093/nar/gkg500
pubmed: 12824352
Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature methods. 9:772. https://doi.org/10.1038/nmeth.2109
doi: 10.1038/nmeth.2109
pubmed: 22847109
pmcid: 4594756
Dewi K (2015) Taxonomical and faunistic studies on the nematode parasites from Indonesian murines (Rodentia; Muridae; Murinae) with special reference to Syphacia spp. and their biogeography. Thesis, Rakuno Gakuen University, Japan
Feldman SH, Bowman SG (2007) Molecular phylogeny of the pinworms of mice, rats and rabbits, and its use to develop molecular beacon assays for the detection of pinworms in mice. Lab Anim 36:43–50. https://doi.org/10.1038/laban1007-43
doi: 10.1038/laban1007-43
Ford C, Hamerton JL (1956) A colchicine hypotonic citrate, squash sequence for mammalian chromosomes. Stain Tech 31:247–251. https://doi.org/10.3109/10520295609113814
doi: 10.3109/10520295609113814
Golden AM, Noffsinger EM, Baldwin JG, Fortuner R, Geraert E, Luc M, Robbins RT (1989) Suggested format for taxonomic papers published in the Journal of Nematology. J Nematology 21:444–445
Goüy de Bellocq J, Ribas A, Baird SJE (2012) New insights into parasitism in the house mouse hybrid zone. In: Macholán M, Baird SJE, Munclinger P, Pialek J (eds) Evolution of the house mouse. Cambridge University Press, Cambridge, pp 455–481
doi: 10.1017/CBO9781139044547.020
Goüy de Bellocq J, Wasimuddin RA, Bryja J, Piálek J, Baird SJE (2018) Holobiont suture zones: parasite evidence across the European house mouse hybrid zone. Mol Ecol 27:5214–5227. https://doi.org/10.1111/mec.14938
doi: 10.1111/mec.14938
pubmed: 30427096
Griffin C, Joyce SA, Dix I, Burnell A, Downes M (1994) Characterisation of the entomopathogenic nematode Heterorhabditis (Nematoda: Heterorhabditidae) from Ireland and Britain by molecular and cross-breeding techniques, and the occurrence of the genus in these islands. Fundam and Appl Nematology 17:245–253
Hugot J (1991) Les Syphaciinae (Oxyuridae, Nematoda) parasites de rongeurs et de lagomorphes: un exemple de co-évolution hôte-parasite. Bulletin de la Société zoologique de France 116:57–62
Kang S, Sultana T, Eom KS, Park YC, Soonthornpong N, Nadler SA, Park JK (2009) The mitochondrial genome sequence of Enterobius vermicularis (Nematoda: Oxyurida)—an idiosyncratic gene order and phylogenetic information for chromadorean nematodes. Gene 429:87–97
doi: 10.1016/j.gene.2008.09.011
Lavrenchenko LA, Potapov SG, Bulatova NS, Golenishchev FN (2009) A genetic study of natural hybridization between two forms of the common vole (Microtus arvalis) with the use of molecular and cytogenetic methods. Dokl Biol Sci 426:222–224. https://doi.org/10.1134/s0012496609030090
doi: 10.1134/s0012496609030090
pubmed: 19650322
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings 2010 gateway computing environments workshop (GCE) http://www.phylo.org/sub_sections/portal/cite.php . Accessed 3 Feb 2020
Nieberding CM, Olivieri I (2007) Parasites: proxies for host genealogy and ecology. Trends in Ecol & Evol 22:156–165. https://doi.org/10.1016/j.tree.2006.11.012
doi: 10.1016/j.tree.2006.11.012
Ogden CG (1971) Observations on the systematics of nematodes belonging to the genus Syphacia Seurat, 1916. Bulletin of the British Museum, Natural History. Zoology. 20:253–280
Okamoto M, Urushima H, Iwasa M, Hasegawa H (2007) Phylogenetic relationships of rodent pinworms (genus Syphacia) in Japan inferred from mitochondrial CO1 gene sequences. J Vet Med Sci 69:545–547. https://doi.org/10.1292/jvms.69.545
doi: 10.1292/jvms.69.545
pubmed: 17551231
Okamoto M, Urushima H, Hasegawa H (2009) Phylogenetic relationships of rodent pinworms (genus Syphacia) in Japan inferred from 28S rDNA sequences. Parasitol Int 58:330–333
doi: 10.1016/j.parint.2009.07.001
Parel JDC, Galula JU, Ooi HK (2008) Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification. Vet Parasitol 153:379–383. https://doi.org/10.1016/j.vetpar.2008.02.001
doi: 10.1016/j.vetpar.2008.02.001
pubmed: 18374491
Ryzhikov KM (ed) (1979) Key to the helminth species of rodents of USSR fauna: nematodes and acanthocephals. Nauka, Moscow [In Russian]
Seinhorst JW (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4:67–69
doi: 10.1163/187529259X00381
Shchipanov NA (1987) A universal live trap for small mammals. Zoologicheskii Zhurnal 66:759–761
Stewart A, Lowe A, Smales L, Bajer A, Bradley J, Dwużnik D, Franssen F, Griffith J, Stuart P, Turner C, Zaleśny G, Behnke JM (2017) Parasitic nematodes of the genus Syphacia Seurat, 1916 infecting Muridae in the British Isles, and the peculiar case of Syphacia frederici. Parasitology 145:269–280. https://doi.org/10.1017/S0031182017001470
doi: 10.1017/S0031182017001470
pubmed: 28831960
Velikov VA (2013) Molecular biology. A practical guide. Saratovskiy istochnik, Saratov [In Russian]
Vrain TC, Wakarchuk DA, Levesque AC, Hamilton RI (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15:563–573
Wang C-R, Yan L, Yan Z, Wang W-T, Xu Z, Xu W-W, Ying Z, Si-Qin T, Lu N, Qiao-Cheng C (2015) Genetic variability among Syphacia obvelata isolates from laboratory mice in four different geographical locations of China revealed by sequence analyses of five mitochondrial genes. Mitochondrial DNA 26:213–216. https://doi.org/10.3109/19401736.2014.905861
doi: 10.3109/19401736.2014.905861
pubmed: 24708136
Wasimuddin BJ, Ribas A, Baird SJ, Piálek J, Goüy de Bellocq J (2016) Testing parasite ‘intimacy’: the whipworm Trichuris muris in the European house mouse hybrid zone. Ecol Evol 6:2688–2701. https://doi.org/10.1002/ece3.2022
doi: 10.1002/ece3.2022
pubmed: 27064973
Wiger R, Barus V, Tenora F (1978) Scanning electron microscopic studies on four species of the genus Syphacia (Nematoda, Oxyuridae). Zool Scr 7:25–31. https://doi.org/10.1111/j.1463-6409.1978.tb00586.x
doi: 10.1111/j.1463-6409.1978.tb00586.x
Zelck UE, Bialek R, Weiß M (2011) Molecular phylogenetic analysis of Enterobius vermicularis and development of an 18S ribosomal DNA-targeted diagnostic PCR. J Clin Microbiol 49:1602–1604
doi: 10.1128/JCM.02454-10