Critical contribution of macrophage scavenger receptor 1 to the uptake of nanostructured DNA by immune cells.
CRISPR/Cas9
DNA nanostructure
DNA uptake
Pull-down assay
Scavenger receptor
Journal
Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
06
05
2020
revised:
27
02
2021
accepted:
28
02
2021
pubmed:
29
3
2021
medline:
15
1
2022
entrez:
28
3
2021
Statut:
ppublish
Résumé
Despite the efficient uptake of polypod-like nanostructured DNA, or polypodna, by macrophage-like RAW264.7 and other immune cells, the detailed mechanism has not been fully elucidated. Our previous study using HEK-Blue hTLR9 cells showed that transfection of macrophage scavenger receptor 1 (MSR1) increased the uptake of tetrapod-like structured DNA. Here, we investigated the involvement of MSR1 in the structure-dependent uptake of polypodna. Transfection of MSR1 to HEK-Blue hTLR9 cells pod number-dependently increased the uptake of polypodna, and its knockout in RAW264.7 cells reduced the uptake and subsequent cytokine release. To examine the binding of DNA with MSR1, biotinylated DNA added to RAW264.7 cells was cross-linked with cell surface proteins. Then, MSR1 cross-linked with polypodna, but not with single-stranded DNA. Similar results were obtained with murine primary immune cells. Taken together, MSR1 discriminates between simple and nanostructured DNAs and plays a dominant role in the efficient uptake of polypodna by immune cells.
Identifiants
pubmed: 33774131
pii: S1549-9634(21)00029-0
doi: 10.1016/j.nano.2021.102386
pii:
doi:
Substances chimiques
Msr1 protein, mouse
0
Scavenger Receptors, Class A
0
DNA
9007-49-2
Dextran Sulfate
9042-14-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102386Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.