Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes.

Journal

ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658

Informations de publication

Date de publication:
23 Mar 2021
Historique:
received: 19 11 2020
accepted: 01 03 2021
entrez: 29 3 2021
pubmed: 30 3 2021
medline: 30 3 2021
Statut: epublish

Résumé

One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one-step qRT-PCR is well suited for the diagnosis of COVID-19 and that there are many commercially available one-step qRT-PCR kits in the market, their high cost and unavailability due to airport closures and shipment restriction became a major bottleneck that had driven the desire to produce the key components of such kits locally. Here, we provide a simple, economical, and powerful one-step qRT-PCR kit based on patent-free, specifically tailored versions of Moloney murine leukemia virus reverse transcriptase and

Identifiants

DOI: 10.1021/acsomega.0c05635 PMID: 33778250 PMC: PMC7986002
pubmed: 33778250
doi: 10.1021/acsomega.0c05635
pmc: PMC7986002
doi:

Types de publication

Journal Article

Langues

eng

Pagination

7374-7386

Informations de copyright

© 2021 The Authors. Published by American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

Références

Anal Biochem. 1990 Dec;191(2):396-400
pubmed: 2085185
J Cell Commun Signal. 2008 Jun;2(1-2):25-45
pubmed: 18766469
Nature. 1970 Jun 27;226(5252):1209-11
pubmed: 4316300
J Acquir Immune Defic Syndr (1988). 1990;3(8):817-31
pubmed: 1694894
Clin Microbiol Infect. 2020 Jun;26(6):773-779
pubmed: 32276116
J Virol. 2007 Jan;81(1):20-9
pubmed: 16928755
J Virol. 1975 Apr;15(4):785-97
pubmed: 46924
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508
Lancet. 2020 Feb 15;395(10223):514-523
pubmed: 31986261
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Microbiol Mol Biol Rev. 2005 Dec;69(4):635-64
pubmed: 16339739
Lancet. 2020 Feb 22;395(10224):565-574
pubmed: 32007145
J Clin Virol. 2020 Jul;128:104412
pubmed: 32416600
Trends Biotechnol. 2011 May;29(5):240-50
pubmed: 21377748
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Public Health. 2020 May;182:170-172
pubmed: 32334183
Nat Biotechnol. 2020 Jul;38(7):870-874
pubmed: 32300245
PLoS Pathog. 2017 Nov 30;13(11):e1006698
pubmed: 29190287
Clin Chem. 2020 Jul 1;66(7):975-977
pubmed: 32315390
Nature. 2013 Nov 28;503(7477):535-8
pubmed: 24172901
Nat Protoc. 2019 Oct;14(10):2986-3012
pubmed: 31548639
J Gen Virol. 1998 Dec;79 ( Pt 12):2921-8
pubmed: 9880005
J Virol Methods. 2003 May;109(2):161-70
pubmed: 12711059
J Clin Virol. 2009 Jul;45(3):196-9
pubmed: 19540799
J Bacteriol. 1976 Sep;127(3):1550-7
pubmed: 8432
Virus Res. 2006 Apr;117(1):17-37
pubmed: 16503362
J Biol Chem. 1985 Aug 5;260(16):9326-35
pubmed: 2410413
Nat Rev Microbiol. 2019 Mar;17(3):181-192
pubmed: 30531947
Anal Biochem. 2007 Jan 1;360(1):84-91
pubmed: 17107651
Nucleic Acids Res. 1992 Apr 11;20(7):1487-90
pubmed: 1374554
Curr Biol. 2020 Jun 8;30(11):2196-2203.e3
pubmed: 32416074
J Virol. 1975 Apr;15(4):843-54
pubmed: 46925
Virus Res. 2020 Oct 15;288:198129
pubmed: 32822689
J Chromatogr A. 2020 Jun 21;1621:461051
pubmed: 32268955
Science. 2005 Oct 28;310(5748):676-9
pubmed: 16195424
J Mol Diagn. 2020 Jun;22(6):729-735
pubmed: 32276051

Auteurs

Masateru Takahashi (M)

Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Muhammad Tehseen (M)

Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Rahul Salunke (R)

Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Etsuko Takahashi (E)

Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Sara Mfarrej (S)

Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Mohamed A Sobhy (MA)

Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Fatimah S Alhamlan (FS)

Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia.

Sharif Hala (S)

Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
King Saud Bin Abdulaziz University of Health Sciences, Jeddah 22384, Saudi Arabia.
King Abdullah International Medical Research Centre, Jeddah, Makkah, Ministry of National Guard Health Affairs, Jeddah, Makkah 22384, Saudi Arabia.

Gerardo Ramos-Mandujano (G)

Stem Cell and Regenration Laboratory. Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Ahmed A Al-Qahtani (AA)

Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia.

Fadwa S Alofi (FS)

Infectious Diseases Department, King Fahad Hospital, Madinah 3177, Saudi Arabia.

Afrah Alsomali (A)

King Abdullah Medical Complex (KAMC), Jeddah 23816, Saudi Arabia.

Anwar M Hashem (AM)

Vaccines and Immunotherapy Unit, King Fahd Medical Research Center; King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Asim Khogeer (A)

Plan and Research Department, General Directorate of Health Affairs Makkah Region, MOH Mecca 24321, Saudi Arabia.

Naif A M Almontashiri (NAM)

College of Applied Medical Sciences, Taibah University, Madinah 41311, Saudi Arabia.
Center for Genetics and Inherited Diseases, Taibah University, Madinah 42353, Saudi Arabia.

Jae Man Lee (JM)

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan.

Hiroaki Mon (H)

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan.

Kosuke Sakashita (K)

Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Mo Li (M)

Stem Cell and Regenration Laboratory. Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Takahiro Kusakabe (T)

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan.

Arnab Pain (A)

Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Samir M Hamdan (SM)

Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Classifications MeSH