Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq.
Adenosine
/ genetics
Adenosine Deaminase
/ genetics
Animals
Fetus
/ cytology
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Immunity, Innate
/ genetics
Immunoprecipitation
/ methods
Liver
/ cytology
Mice
RNA
/ analysis
RNA Editing
/ genetics
RNA, Double-Stranded
/ genetics
Transcriptome
/ genetics
Cell Biology
Immunology
Model Organisms
RNA-seq
Sequencing
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
entrez:
29
3
2021
pubmed:
30
3
2021
medline:
30
3
2021
Statut:
epublish
Résumé
Double-stranded RNAs (dsRNAs) are abundantly present in cells, playing multiple regulatory functions. dsRNAs of viral origin activate innate immune responses. Since RNA editing and modifications affect the structure and recognition of RNAs, their alteration can result in the accumulation of aberrant endogenous dsRNAs inducing a deleterious innate immune response. Here, we present a complete protocol for the measurement of dsRNAs in a live mouse tissue using dsRNA immunoprecipitation and sequencing (dsRIP-Seq). This protocol focuses on tissue isolation, dsRNA immunoprecipitation and downstream computational analysis. For complete details on the use and execution of this protocol, please refer to Gao et al. (2020).
Identifiants
pubmed: 33778776
doi: 10.1016/j.xpro.2021.100366
pii: S2666-1667(21)00073-3
pmc: PMC7982789
doi:
Substances chimiques
RNA, Double-Stranded
0
RNA
63231-63-0
Adenosine Deaminase
EC 3.5.4.4
Adenosine
K72T3FS567
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100366Subventions
Organisme : NCI NIH HHS
ID : R01 CA222518
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK106857
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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