Increasing Long-Chain Dicarboxylic Acid Production in Candida tropicalis by Engineering Fatty Transporters.
Candida tropicalis 1798
Ctfat1p
Ctpxa1p
Fatty acid transporters
Long-chain dicarboxylic acids
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
27
10
2020
accepted:
20
03
2021
pubmed:
1
4
2021
medline:
2
9
2021
entrez:
31
3
2021
Statut:
ppublish
Résumé
Candida tropicalis can metabolize alkanes or fatty acids to produce long-chain dicarboxylic acids (DCAs). Fatty acid transporters located on the cell or peroxisome membrane may play an important role in this process. Using amino acid sequence homologous alignment, two putative proteins, CtFat1p and CtPxa1p, located on the cell and peroxisome membrane were found, respectively. Moreover, single- and double-knockout homologous recombination technology was used to study ctfat1p and ctpxa1p gene effects on DCA synthesis. In comparison to the wild-type strain, long-chain DCA yield decreased by 65.14%, 88.38% and 56.19% after single and double-copy knockout of ctfat1p genes and double-copy knockout of ctpxa1p genes, respectively, indicating that the knockout of ctfat1p and ctpxa1p genes had a significant effect on the conversion of oils and fats into long-chain DCAs by C. tropicalis. However, the yield of long-chain DCAs increased by 21.90% after single-knockout of the ctpxa1p gene, indicating that the single-knockout of the ctpxa1p gene may reduce fatty acid transport to peroxisome for further oxidation. Moreover, to improve the intracellular transport rate of fatty acids, ctfat1p copy number increased, increasing DCA yield by 30.10%. These results may provide useful information for enhancing the production of long-chain DCAs by C. tropicalis.
Identifiants
pubmed: 33786739
doi: 10.1007/s12033-021-00319-6
pii: 10.1007/s12033-021-00319-6
doi:
Substances chimiques
Alkanes
0
Fatty Acid Transport Proteins
0
Fatty Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
544-555Subventions
Organisme : National Natural Science Foundation of China
ID : 31801527
Organisme : Focus on Research and Development Plan in Shandong Province
ID : 2019JZZY011003
Organisme : Focus on Research and Development Plan in Shandong Province
ID : 2018YFJH0401
Organisme : National Key Research and Development Project
ID : 2019YFC1905900
Organisme : Taishan industry leading talent
ID : tscy20180103
Organisme : Major Program of National Natural Science Foundation of Shandong
ID : ZR2017ZB0208
Organisme : Synthetic Biology Technology Innovation Center of Shandong Province
ID : sdsynbio-2018-PY-02
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