Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.
Antimitotic Agents
/ toxicity
Antineoplastic Agents
/ toxicity
Calcium-Binding Proteins
/ genetics
Cell Death
Cell Proliferation
Chromosomal Proteins, Non-Histone
/ genetics
Genes, Reporter
Green Fluorescent Proteins
/ genetics
HCT116 Cells
Histones
/ genetics
Humans
Mitosis
Tumor Stem Cell Assay
/ methods
Centrin
Clonogenic assay
Histone
Mitotic catastrophe
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
31
3
2021
pubmed:
1
4
2021
medline:
17
6
2021
Statut:
ppublish
Résumé
Mitotic catastrophe (MC) is a cell death modality induced by DNA damage that involves the activation of cell cycle checkpoints such as the "DNA structure checkpoint" and "spindle assembly checkpoint" (SAC) leading to aberrant mitosis. Depending on the signal, MC can drive the cell to death or to senescence. The suppression of MC favors aneuploidy. Several cancer therapies, included microtubular poisons and radiations, trigger MC. The clonogenic assay has been used to study the capacity of single cells to proliferate and to generate macroscopic colonies and to evaluate the efficacy of anticancer drugs. Nevertheless, this method cannot analyze MC events. Here, we report an improved technique based on the use of human colon cancer HCT116 stable expressing histone H2B-GFP and DsRed-centrin proteins, allowing to determine the capacity of cells to proliferate, and to determine changes in the nucleus and centrosomes.
Identifiants
pubmed: 33786796
doi: 10.1007/978-1-0716-1217-0_16
doi:
Substances chimiques
Antimitotic Agents
0
Antineoplastic Agents
0
Calcium-Binding Proteins
0
Chromosomal Proteins, Non-Histone
0
Histones
0
caltractin
118216-31-2
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-239Références
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