Amazonian mammal monitoring using aquatic environmental DNA.
Amazonian mammals
aquatic eDNA
metabarcoding
monitoring
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
14
02
2021
received:
18
11
2020
accepted:
22
03
2021
pubmed:
1
4
2021
medline:
20
8
2021
entrez:
31
3
2021
Statut:
ppublish
Résumé
Environmental DNA (eDNA) metabarcoding has emerged as one of the most efficient methods to assess aquatic species presence. While the method can in theory be used to investigate nonaquatic fauna, its development for inventorying semi-aquatic and terrestrial fauna is still at an early stage. Here we investigated the potential of aquatic eDNA metabarcoding for inventorying mammals in Neotropical environments, be they aquatic, semi-aquatic or terrestrial. We collected aquatic eDNA in 96 sites distributed along three Guianese watersheds and compared our inventories to expected species distributions and field observations derived from line transects located throughout French Guiana. Species occurrences and emblematic mammalian fauna richness patterns were consistent with the expected distribution of fauna and our results revealed that aquatic eDNA metabarcoding brings additional data to line transect samples for diurnal nonaquatic (terrestrial and arboreal) species. Aquatic eDNA also provided data on species not detectable in line transect surveys such as semi-aquatic, aquatic and nocturnal terrestrial and arboreal species. Although the application of eDNA to inventory mammals still needs some developments to optimize sampling efficiency, it can now be used as a complement to traditional surveys.
Identifiants
pubmed: 33787010
doi: 10.1111/1755-0998.13393
doi:
Substances chimiques
DNA, Environmental
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1875-1888Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-10-LABX-25-01
Organisme : Agence Nationale de la Recherche
ID : ANR-10-LABX-0041
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABX-0010
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE02-0007-01
Organisme : Observatoire Homme-Milieu Oyapock
ID : ANR-11-LABX-0010
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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