Streptococcus pyogenes upregulates arginine catabolism to exert its pathogenesis on the skin surface.
Animals
Arginine
/ metabolism
Bacterial Proteins
/ genetics
Filaggrin Proteins
Gene Expression Regulation, Bacterial
HaCaT Cells
Humans
Hydrolases
/ metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
Microbial Viability
Phosphorylation
Skin
/ microbiology
Streptococcal Infections
/ blood
Streptococcus pyogenes
/ genetics
Transcriptome
/ genetics
Up-Regulation
Virulence
CovR phosphorylation
Streptococcus pyogenes
arginine
arginine deiminase pathway
bacterial pathogenesis
bacterial viability
filaggrin
glucose starvation
pyroptosis
skin infection
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
30 03 2021
30 03 2021
Historique:
received:
17
06
2020
revised:
15
01
2021
accepted:
09
03
2021
entrez:
31
3
2021
pubmed:
1
4
2021
medline:
27
1
2022
Statut:
ppublish
Résumé
The arginine deiminase (ADI) pathway has been found in many kinds of bacteria and functions to supplement energy production and provide protection against acid stress. The Streptococcus pyogenes ADI pathway is upregulated upon exposure to various environmental stresses, including glucose starvation. However, there are several unclear points about the advantages to the organism for upregulating arginine catabolism. We show that the ADI pathway contributes to bacterial viability and pathogenesis under low-glucose conditions. S. pyogenes changes global gene expression, including upregulation of virulence genes, by catabolizing arginine. In a murine model of epicutaneous infection, S. pyogenes uses the ADI pathway to augment its pathogenicity by increasing the expression of virulence genes, including those encoding the exotoxins. We also find that arginine from stratum-corneum-derived filaggrin is a key substrate for the ADI pathway. In summary, arginine is a nutrient source that promotes the pathogenicity of S. pyogenes on the skin.
Identifiants
pubmed: 33789094
pii: S2211-1247(21)00238-2
doi: 10.1016/j.celrep.2021.108924
pmc: PMC9214650
mid: NIHMS1808547
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Filaggrin Proteins
0
Arginine
94ZLA3W45F
Hydrolases
EC 3.-
arginine deiminase
EC 3.5.3.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108924Subventions
Organisme : NIAID NIH HHS
ID : R37 AI052453
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI096837
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI077780
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145325
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI140436
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no conflict of interest.
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