Oxytocin in bovine saliva: validation of two assays and changes in parturition and at weaning.
Calves
Cow
Oxytocin
Saliva
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
01 Apr 2021
01 Apr 2021
Historique:
received:
19
12
2020
accepted:
15
03
2021
entrez:
2
4
2021
pubmed:
3
4
2021
medline:
26
8
2021
Statut:
epublish
Résumé
The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated. Also, the changes in oxytocin in saliva were explored in two different situations. One was around parturition, and for this purpose, saliva samples from 13 cows were collected at three different times: 7 days before the parturition, the day of parturition and 7 days after the parturition. The second situation was weaning and grouping of calves, and for this purpose, saliva from 25 calves was collected at three different times: 1 day before weaning, 2 days after weaning or milk withdrawal and 4 days after grouping calves. In cows, oxytocin concentrations showed an increase on the day of parturition with both assays, while in calves, oxytocin concentrations showed a decrease 4 days after the grouping. The assays validated in this report could be used for the measurement of oxytocin in bovine saliva and detect changes in this analyte that can occur in different physiological or productive situations such as parturition and weaning.
Sections du résumé
BACKGROUND
BACKGROUND
The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated. Also, the changes in oxytocin in saliva were explored in two different situations. One was around parturition, and for this purpose, saliva samples from 13 cows were collected at three different times: 7 days before the parturition, the day of parturition and 7 days after the parturition. The second situation was weaning and grouping of calves, and for this purpose, saliva from 25 calves was collected at three different times: 1 day before weaning, 2 days after weaning or milk withdrawal and 4 days after grouping calves.
RESULTS
RESULTS
In cows, oxytocin concentrations showed an increase on the day of parturition with both assays, while in calves, oxytocin concentrations showed a decrease 4 days after the grouping.
CONCLUSIONS
CONCLUSIONS
The assays validated in this report could be used for the measurement of oxytocin in bovine saliva and detect changes in this analyte that can occur in different physiological or productive situations such as parturition and weaning.
Identifiants
pubmed: 33794896
doi: 10.1186/s12917-021-02838-5
pii: 10.1186/s12917-021-02838-5
pmc: PMC8017845
doi:
Substances chimiques
Oxytocin
50-56-6
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
140Subventions
Organisme : Ministerio de Educación, Cultura y Deporte
ID : FPU16/02170
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : IJC2018-035105-I
Organisme : Ministerio de Economía y Competitividad
ID : AGL2016-79096-R
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