The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII.
Arabidopsis
Chlorophyll
/ metabolism
Hydrogen-Ion Concentration
Light
Light-Harvesting Protein Complexes
/ chemistry
Membrane Proteins
/ chemistry
Photosynthesis
Photosystem II Protein Complex
/ chemistry
Plant Physiological Phenomena
Proteolipids
/ chemistry
Spectrum Analysis
Thylakoids
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 04 2021
01 04 2021
Historique:
received:
08
01
2021
accepted:
11
03
2021
entrez:
2
4
2021
pubmed:
3
4
2021
medline:
23
11
2021
Statut:
epublish
Résumé
Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.
Identifiants
pubmed: 33795805
doi: 10.1038/s41598-021-86975-9
pii: 10.1038/s41598-021-86975-9
pmc: PMC8016914
doi:
Substances chimiques
Light-Harvesting Protein Complexes
0
Membrane Proteins
0
Photosystem II Protein Complex
0
Proteolipids
0
proteoliposomes
0
Chlorophyll
1406-65-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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