Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts.
Biolistics
Capripoxvirus
/ genetics
Chloroplasts
/ genetics
Chromatography, Affinity
Codon, Terminator
Plant Leaves
/ metabolism
Plants, Genetically Modified
/ growth & development
Promoter Regions, Genetic
Protein Engineering
Recombinant Proteins
/ metabolism
Nicotiana
/ genetics
Viral Structural Proteins
/ genetics
SPPV117 protein
Sheep pox virus
Transplastomic plants
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
04
10
2020
accepted:
15
03
2021
pubmed:
3
4
2021
medline:
15
12
2021
entrez:
2
4
2021
Statut:
ppublish
Résumé
A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox. Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox.
Identifiants
pubmed: 33797655
doi: 10.1007/s10529-021-03117-x
pii: 10.1007/s10529-021-03117-x
pmc: PMC8017516
doi:
Substances chimiques
Codon, Terminator
0
Recombinant Proteins
0
Viral Structural Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1475-1485Subventions
Organisme : Ministry of Education and Science of the Republic of Kazakhstan
ID : APO5132066
Organisme : Ministry of Education and Science of the Republic of Kazakhstan
ID : AP08855746
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