The Combination of Bromelain and Acetylcysteine (BromAc) Synergistically Inactivates SARS-CoV-2.
Acetylcysteine
BromAc
Bromelain
SARS-CoV-2
drug repurposing
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
06 03 2021
06 03 2021
Historique:
received:
31
01
2021
revised:
25
02
2021
accepted:
01
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
20
4
2021
Statut:
epublish
Résumé
Severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection is the cause of a worldwide pandemic, currently with limited therapeutic options. The spike glycoprotein and envelope protein of SARS-CoV-2, containing disulfide bridges for stabilization, represent an attractive target as they are essential for binding to the ACE2 receptor in host cells present in the nasal mucosa. Bromelain and Acetylcysteine (BromAc) has synergistic action against glycoproteins by breakage of glycosidic linkages and disulfide bonds. We sought to determine the effect of BromAc on the spike and envelope proteins and its potential to reduce infectivity in host cells. Recombinant spike and envelope SARS-CoV-2 proteins were disrupted by BromAc. Spike and envelope protein disulfide bonds were reduced by Acetylcysteine. In in vitro whole virus culture of both wild-type and spike mutants, SARS-CoV-2 demonstrated a concentration-dependent inactivation from BromAc treatment but not from single agents. Clinical testing through nasal administration in patients with early SARS-CoV-2 infection is imminent.
Identifiants
pubmed: 33800932
pii: v13030425
doi: 10.3390/v13030425
pmc: PMC7999995
pii:
doi:
Substances chimiques
Antiviral Agents
0
Spike Glycoprotein, Coronavirus
0
Bromelains
9001-00-7
Acetylcysteine
WYQ7N0BPYC
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Mucpharm pty ltd
ID : COVID research
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