Development of a Point-of-Care Assay for HIV-1 Viral Load Using Higher Refractive Index Antibody-Coated Microbeads.
HIV-1
computational analysis
lensless imaging
point-of-care diagnosis
portable systems
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
05 Mar 2021
05 Mar 2021
Historique:
received:
30
01
2021
revised:
26
02
2021
accepted:
28
02
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
28
4
2021
Statut:
epublish
Résumé
The detection of viruses using imaging techniques is challenging because of the weak scattering of light generated by the targets of sizes in the nanometer range. The system we have developed overcomes the light scattering problems by utilizing antibody-coated microbeads of higher index of refraction that can specifically bind with viruses and increase the acceptance angle. Using the new technology, we have developed a portable, cost-effective, and field-deployable platform for the rapid quantification of HIV-1 viral load for point-of-care (POC) settings. The system combines microfluidics with a wide field of view lensless imaging technology. Highly specific antibodies are functionalized to a glass slide inside a microchip to capture HIV-1 virions. The captured virions are then bound by antibody-conjugated microbeads, which have a higher refraction index. The microbeads-HIV-1 virions complexes generate diffraction patterns that are detected with a custom-built imaging setup and rapidly and accurately quantified by computational analysis. This platform technology enables fast nanoscale virus imaging and quantification from biological samples and thus can play a significant role in the detection and management of viral diseases.
Identifiants
pubmed: 33807789
pii: s21051819
doi: 10.3390/s21051819
pmc: PMC7961362
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : R56AI138659
Pays : United States
Organisme : National Science Foundation
ID : NSF CAREER Award 1942487
Organisme : Humanity in Science Award
ID : Humanity in Science Award
Références
Biol Cell. 2008 Aug;100(8):491-501
pubmed: 18627353
ACS Omega. 2019 Apr 30;4(4):7474-7481
pubmed: 31080939
Annu Rev Med. 2015;66:387-405
pubmed: 25423597
JAMA. 1997 Jul 2;278(1):58-62
pubmed: 9207340
Infect Disord Drug Targets. 2012 Oct;12(5):346-56
pubmed: 23017163
Antiviral Res. 2010 Jan;85(1):1-18
pubmed: 20018391
Science. 1987 Sep 11;237(4820):1351-5
pubmed: 3629244
Nat Commun. 2020 Jan 16;11(1):171
pubmed: 31949134
Sci Rep. 2018 May 16;8(1):7653
pubmed: 29769566
EMBO J. 2003 Apr 1;22(7):1707-15
pubmed: 12660176
Sci Rep. 2018 Sep 19;8(1):14303
pubmed: 30232388
Biosens Bioelectron. 2009 Sep 15;25(1):253-8
pubmed: 19665685
Biosens Bioelectron. 2020 Oct 1;165:112417
pubmed: 32729535
Clin Microbiol Rev. 2009 Oct;22(4):552-63
pubmed: 19822888
PLoS One. 2017 Jun 26;12(6):e0179728
pubmed: 28651016
Nat Med. 2003 Jul;9(7):847-52
pubmed: 12835704
Opt Lasers Eng. 2021 Feb;137:
pubmed: 32905530
J Acquir Immune Defic Syndr. 2021 Feb 1;86(2):258-263
pubmed: 33136821
Nano Lett. 2010 Nov 10;10(11):4727-31
pubmed: 20964282
IEEE Rev Biomed Eng. 2013;6:29-46
pubmed: 23558399
RSC Adv. 2019;9(15):8159-8168
pubmed: 31777654
Clin Chim Acta. 2020 Feb;501:85-91
pubmed: 31678569
Crit Rev Biotechnol. 2020 Aug;40(5):623-638
pubmed: 32308063
Diagnostics (Basel). 2020 Jan 14;10(1):
pubmed: 31947549
Nat Commun. 2017 Sep 15;8(1):545
pubmed: 28916807
Sci Rep. 2015 Mar 06;5:8719
pubmed: 25743880
Talanta. 2013 Oct 15;115:68-73
pubmed: 24054563
Front Microbiol. 2019 Jan 07;9:3255
pubmed: 30666247
Lab Chip. 2013 Jun 7;13(11):2028-35
pubmed: 23592185
Biosens Bioelectron. 2020 Apr 1;153:112041
pubmed: 31999560
Rev Med Virol. 2019 Jan;29(1):e2019
pubmed: 30411832
Retrovirology. 2018 Jun 8;15(1):41
pubmed: 29884197
Arch Pathol Lab Med. 2020 Nov 1;144(11):1335-1343
pubmed: 32886758
Nat Struct Mol Biol. 2004 Jul;11(7):672-5
pubmed: 15208690
J Clin Virol. 1999 Jun;13(1-2):105-19
pubmed: 10405897
Lab Chip. 2017 Aug 22;17(17):2910-2919
pubmed: 28702612
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7296-301
pubmed: 21504943
Biosens Bioelectron. 2019 Oct 1;142:111490
pubmed: 31302394
J Clin Microbiol. 2005 Jun;43(6):2709-17
pubmed: 15956387
Small. 2013 Aug 12;9(15):2553-63, 2478
pubmed: 23447456
Lancet. 2010 Jul 3;376(9734):49-62
pubmed: 20609987
Expert Rev Mol Diagn. 2017 Apr;17(4):351-366
pubmed: 28103450
Virology. 1973 Dec;56(2):652-3
pubmed: 4796553
Structure. 2000 Apr 15;8(4):385-95
pubmed: 10801487
N Engl J Med. 1991 Apr 4;324(14):961-4
pubmed: 1823118
N Engl J Med. 2011 May 19;364(20):1943-54
pubmed: 21591946
Nucleic Acids Res. 2002 Mar 15;30(6):1292-305
pubmed: 11884626
Chem Rev. 2020 Feb 12;120(3):1936-1979
pubmed: 31951121
Eur Biophys J. 2008 Oct;37(8):1291-301
pubmed: 18401579
AIDS Res Treat. 2014;2014:625082
pubmed: 24895531
Lab Chip. 2010 Jun 7;10(11):1417-28
pubmed: 20401422
J Clin Virol. 2001 Aug;22(1):1-9
pubmed: 11418348