The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in nonhuman primates.
Journal
Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086
Informations de publication
Date de publication:
12 05 2021
12 05 2021
Historique:
received:
09
10
2020
revised:
19
01
2021
accepted:
31
03
2021
pubmed:
7
4
2021
medline:
24
6
2021
entrez:
6
4
2021
Statut:
ppublish
Résumé
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) poses a public health threat for which preventive and therapeutic agents are urgently needed. Neutralizing antibodies are a key class of therapeutics that may bridge widespread vaccination campaigns and offer a treatment solution in populations less responsive to vaccination. Here, we report that high-throughput microfluidic screening of antigen-specific B cells led to the identification of LY-CoV555 (also known as bamlanivimab), a potent anti-spike neutralizing antibody from a hospitalized, convalescent patient with coronavirus disease 2019 (COVID-19). Biochemical, structural, and functional characterization of LY-CoV555 revealed high-affinity binding to the receptor-binding domain, angiotensin-converting enzyme 2 binding inhibition, and potent neutralizing activity. A pharmacokinetic study of LY-CoV555 conducted in cynomolgus monkeys demonstrated a mean half-life of 13 days and a clearance of 0.22 ml hour
Identifiants
pubmed: 33820835
pii: scitranslmed.abf1906
doi: 10.1126/scitranslmed.abf1906
pmc: PMC8284311
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Spike Glycoprotein, Coronavirus
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : U01 AI047996
Pays : United States
Organisme : NIAID NIH HHS
ID : F32 AI152296
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : T32 AI007151
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI157155
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI139374
Pays : United States
Organisme : NIAID NIH HHS
ID : UC7 AI094660
Pays : United States
Organisme : NIH HHS
ID : AI157155
Pays : United States
Organisme : NIH HHS
ID : UM1AI48574
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI143639
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI148574
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
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