MicroRNA-29 is an essential regulator of brain maturation through regulation of CH methylation.

3' Untranslated Regions / genetics Animals Animals, Newborn Base Sequence Behavior, Animal Brain / growth & development DNA (Cytosine-5-)-Methyltransferases / metabolism DNA Methylation / genetics Down-Regulation / genetics Gene Expression Regulation, Developmental Mice, Inbred C57BL MicroRNAs / genetics Neurodevelopmental Disorders / genetics Neurons / metabolism Seizures / genetics Signal Transduction Synapses / metabolism Up-Regulation / genetics
CH methylation DNMT3A MeCP2 autism epilepsy miR-29 miRNA neurodevelopmental disorders non-CG methylation seizures

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
06 04 2021
Historique:
received: 28 07 2020
revised: 09 12 2020
accepted: 14 03 2021
entrez: 7 4 2021
pubmed: 8 4 2021
medline: 27 1 2022
Statut: ppublish

Résumé

Although embryonic brain development and neurodegeneration have received considerable attention, the events that govern postnatal brain maturation are less understood. Here, we identify the miR-29 family to be strikingly induced during the late stages of brain maturation. Brain maturation is associated with a transient, postnatal period of de novo non-CG (CH) DNA methylation mediated by DNMT3A. We examine whether an important function of miR-29 during brain maturation is to restrict the period of CH methylation via its targeting of Dnmt3a. Deletion of miR-29 in the brain, or knockin mutations preventing miR-29 to specifically target Dnmt3a, result in increased DNMT3A expression, higher CH methylation, and repression of genes associated with neuronal activity and neuropsychiatric disorders. These mouse models also develop neurological deficits and premature lethality. Our results identify an essential role for miR-29 in restricting CH methylation in the brain and illustrate the importance of CH methylation regulation for normal brain maturation.

Identifiants

DOI: 10.1016/j.celrep.2021.108946 PMID: 33826889 PMC: PMC8103628
pubmed: 33826889
pii: S2211-1247(21)00260-6
doi: 10.1016/j.celrep.2021.108946
pmc: PMC8103628
mid: NIHMS1691239
pii:
doi:

Substances chimiques

3' Untranslated Regions 0
MIRN29 microRNA, mouse 0
MicroRNAs 0
DNA (Cytosine-5-)-Methyltransferases EC 2.1.1.37

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108946

Subventions

Organisme : NCI NIH HHS
ID : U54 CA156733
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES010126
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118331
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD103573
Pays : United States
Organisme : NINDS NIH HHS
ID : P30 NS045892
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS116859
Pays : United States
Organisme : NIEHS NIH HHS
ID : P42 ES031007
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD098657
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016086
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD079124
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG055304
Pays : United States

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Vijay Swahari (V)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA. Electronic address: swahari@email.unc.edu.

Ayumi Nakamura (A)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA; Neurobiology Curriculum, University of North Carolina, Chapel Hill, NC, USA.

Emilie Hollville (E)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA.

Hume Stroud (H)

Department of Neurobiology, Harvard University, Boston, MA, USA.

Jeremy M Simon (JM)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA; Department of Genetics, University of North Carolina, Chapel Hill, NC, USA; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, NC, USA.

Travis S Ptacek (TS)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, NC, USA.

Matthew V Beck (MV)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA.

Cornelius Flowers (C)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA.

Jiami Guo (J)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA.

Charlotte Plestant (C)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA.

Jie Liang (J)

Department of Immunology, Duke University, Durham, NC, USA.

C Lisa Kurtz (CL)

Department of Genetics, University of North Carolina, Chapel Hill, NC, USA.

Matt Kanke (M)

Department of Biomedical Sciences, Cornell University, Ithaca, NY, USA.

Scott M Hammond (SM)

Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC, USA.

You-Wen He (YW)

Department of Immunology, Duke University, Durham, NC, USA.

E S Anton (ES)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA; Neurobiology Curriculum, University of North Carolina, Chapel Hill, NC, USA; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC, USA.

Praveen Sethupathy (P)

Department of Genetics, University of North Carolina, Chapel Hill, NC, USA; Department of Biomedical Sciences, Cornell University, Ithaca, NY, USA.

Sheryl S Moy (SS)

Department of Psychiatry, University of North Carolina, Chapel Hill, NC, USA; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, NC, USA.

Michael E Greenberg (ME)

Department of Neurobiology, Harvard University, Boston, MA, USA.

Mohanish Deshmukh (M)

Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA; Neurobiology Curriculum, University of North Carolina, Chapel Hill, NC, USA; Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, NC, USA; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC, USA. Electronic address: mohanish@med.unc.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Régions non traduites › Régions 3' non traduites › MicroRNA-29 is an essential regulator of brain...
questionsmedicales.fr › Eucaryotes › Animaux › MicroRNA-29 is an essential regulator of brain...
questionsmedicales.fr › Eucaryotes › Animaux › Groupes animaux › Animaux nouveau-nés › MicroRNA-29 is an essential regulator of brain...
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questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › One-carbon group transferases › Methyltransferases › DNA modification methylases › DNA-Cytosine Methylases › DNA (cytosine-5-)-methyltransferase › MicroRNA-29 is an essential regulator of brain...
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