Immunophenotyping of Peripheral Blood Mononuclear Cells in Septic Shock Patients With High-Dimensional Flow Cytometry Analysis Reveals Two Subgroups With Differential Responses to Immunostimulant Drugs.
Antigens, CD
/ pharmacology
Biomarkers
/ blood
Case-Control Studies
Cells, Cultured
Cytokines
/ blood
Flow Cytometry
HLA-DR Antigens
/ blood
Humans
Immunophenotyping
Interferon-gamma
/ pharmacology
Leukocytes, Mononuclear
/ drug effects
Lipopolysaccharides
/ pharmacology
Monitoring, Immunologic
Phenotype
Predictive Value of Tests
Shock, Septic
/ blood
Workflow
Lymphocyte Activation Gene 3 Protein
LAG-3Ig
Lipopolysaccharides
high-dimensional flow cytometry
immunophenotype
interferon-γ
sepsis
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
27
11
2020
accepted:
22
02
2021
entrez:
8
4
2021
pubmed:
9
4
2021
medline:
22
9
2021
Statut:
epublish
Résumé
Sepsis is associated with a dysregulated inflammatory response to infection. Despite the activation of inflammation, an immune suppression is often observed, predisposing patients to secondary infections. Therapies directed at restoration of immunity may be considered but should be guided by the immune status of the patients. In this paper, we described the use of a high-dimensional flow cytometry (HDCyto) panel to assess the immunophenotype of patients with sepsis. We then isolated peripheral blood mononuclear cells (PBMCs) from patients with septic shock and mimicked a secondary infection by stimulating PBMCs for 4 h
Identifiants
pubmed: 33828550
doi: 10.3389/fimmu.2021.634127
pmc: PMC8019919
doi:
Substances chimiques
Antigens, CD
0
Biomarkers
0
Cytokines
0
HLA-DR Antigens
0
IFNG protein, human
0
Lipopolysaccharides
0
Interferon-gamma
82115-62-6
Lymphocyte Activation Gene 3 Protein
0
Lag3 protein, human
0
soluble LAG-3 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
634127Informations de copyright
Copyright © 2021 Wang, Gloss, Tang, Dervish, Santner-Nanan, Whitehead, Masters, Skarratt, Teoh, Schibeci, Fewings, Brignone, Triebel, Booth, McLean and Nalos.
Déclaration de conflit d'intérêts
CB and BT were employed by Immutep S.A.S. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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