Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation.
COVID-19
SARS-CoV-2
airway organoids
cell culture adaptation
furin cleavage site
infectious disease
microbiology
serine proteases
virus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
09 04 2021
09 04 2021
Historique:
received:
22
01
2021
accepted:
08
04
2021
pubmed:
10
4
2021
medline:
28
5
2021
entrez:
9
4
2021
Statut:
epublish
Résumé
Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 - that expresses serine proteases - prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation.
Identifiants
pubmed: 33835028
doi: 10.7554/eLife.66815
pii: 66815
pmc: PMC8131099
doi:
pii:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Serine Proteases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Netherlands Organization for Health Research and Development
ID : 10150062010008
Organisme : PPP allowance
ID : LSHM19136
Organisme : ZonMw
ID : 10150062010008
Pays : Netherlands
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021, Lamers et al.
Déclaration de conflit d'intérêts
ML, AM, TB, YW, DW, SR, Pv, DS, TB, NW, BH No competing interests declared
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