Phytochemical analysis of medicinal plants of Nepal and their antibacterial and antibiofilm activities against uropathogenic Escherichia coli.

Anti-Infective Agents / pharmacology Biofilms / drug effects Nepal Plants, Medicinal / chemistry Uropathogenic Escherichia coli / drug effects
Antibacterial activity Antibiofilm activity Biofilm Medicinal plants

Journal

BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232

Informations de publication

Date de publication:
09 Apr 2021
Historique:
received: 27 08 2020
accepted: 30 03 2021
entrez: 10 4 2021
pubmed: 11 4 2021
medline: 25 11 2021
Statut: epublish

Résumé

A biofilm is an extracellular polymeric substance (EPS) composed of polysaccharides, proteins, nucleic acids, and lipids that impede antibiotics and immune cells, thus providing a shielded environment for bacterial growth. Due to biofilm formation, some microbes can show up to 1000 fold increased resistance towards the antibiotics than the normal planktonic forms. The study was conducted to screen the crude extracts of medicinal plants used in Nepal for their in vitro antibiofilm activities. Total phenolic and total flavonoid contents were determined by using a Folin-Ciocalteau reagent and aluminium trichloride method, respectively. Resazurin assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The initial antibiofilm activities and their inhibitory concentration (IC Out of 25 different plant extracts were used for the study, methanolic extracts of 20 plants showed a biofilm inhibition activity against five different strong biofilm producing Escherichia coli strains. Calotropis gigantea exhibited inhibition against all five different E. coli strains with IC Extracts of C. gigantea, E. prostrata, P. persica, U. parviflora, and O. tenuifolium showed antibacterial as well as antibiofilm activity against pathogenic and strong biofilm producing E. coli. Thus, extracts or the pure compound from these medicinal plants could be used as antibiotics in the future.

Sections du résumé

BACKGROUND BACKGROUND
A biofilm is an extracellular polymeric substance (EPS) composed of polysaccharides, proteins, nucleic acids, and lipids that impede antibiotics and immune cells, thus providing a shielded environment for bacterial growth. Due to biofilm formation, some microbes can show up to 1000 fold increased resistance towards the antibiotics than the normal planktonic forms. The study was conducted to screen the crude extracts of medicinal plants used in Nepal for their in vitro antibiofilm activities.
METHODS METHODS
Total phenolic and total flavonoid contents were determined by using a Folin-Ciocalteau reagent and aluminium trichloride method, respectively. Resazurin assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The initial antibiofilm activities and their inhibitory concentration (IC
RESULTS RESULTS
Out of 25 different plant extracts were used for the study, methanolic extracts of 20 plants showed a biofilm inhibition activity against five different strong biofilm producing Escherichia coli strains. Calotropis gigantea exhibited inhibition against all five different E. coli strains with IC
CONCLUSION CONCLUSIONS
Extracts of C. gigantea, E. prostrata, P. persica, U. parviflora, and O. tenuifolium showed antibacterial as well as antibiofilm activity against pathogenic and strong biofilm producing E. coli. Thus, extracts or the pure compound from these medicinal plants could be used as antibiotics in the future.

Identifiants

DOI: 10.1186/s12906-021-03293-3 PMID: 33836728 PMC: PMC8033659
pubmed: 33836728
doi: 10.1186/s12906-021-03293-3
pii: 10.1186/s12906-021-03293-3
pmc: PMC8033659
doi:

Substances chimiques

Anti-Infective Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

116

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Auteurs

Sudip Bhandari (S)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Karan Khadayat (K)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Sami Poudel (S)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Sunil Shrestha (S)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Raju Shrestha (R)

Department of Microbiology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Poonam Devkota (P)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Santosh Khanal (S)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal.

Bishnu P Marasini (BP)

Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, Nepal. bishnu.marasini@gmail.com.
ORCID: 0000-0001-6153-5234

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Phytochemical analysis of medicinal plants of...
questionsmedicales.fr › Phénomènes microbiologiques › Biofilms › Phytochemical analysis of medicinal plants of...
questionsmedicales.fr › Régions géographiques › Asie › Asie de l'Ouest › Asie du Sud › Népal › Phytochemical analysis of medicinal plants of...
questionsmedicales.fr › Eucaryotes › Plantes › Plantes médicinales › Phytochemical analysis of medicinal plants of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli pathogènes extra-intestinales › Escherichia coli uropathogène › Phytochemical analysis of medicinal plants of...