First detection of porcine respirovirus 1 in Germany and the Netherlands.
Europe
next-generation sequencing
porcine parainfluenza virus 1
porcine respirovirus 1
shotgun metagenomics sequencing
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
10
03
2021
received:
10
11
2020
accepted:
06
04
2021
pubmed:
11
4
2021
medline:
26
11
2021
entrez:
10
4
2021
Statut:
ppublish
Résumé
Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch-German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.
Identifiants
pubmed: 33837672
doi: 10.1111/tbed.14100
pmc: PMC9292642
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3120-3125Subventions
Organisme : European Regional Development Fund
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 713660
Informations de copyright
© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH.
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