Epidemiology of
Kala-azar
Leishmania
host
nested PCR
rk39
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
12
2020
accepted:
10
03
2021
entrez:
12
4
2021
pubmed:
13
4
2021
medline:
6
7
2021
Statut:
epublish
Résumé
Leishmaniasis is a regional infectious disease caused by the bite of The rK-39 strip test was performed on blood samples from previously infected patients. Blood samples were collected from the patients and their families. The blood, liver, spleen, and diaphragm muscle samples were collected from livestock. To perform nested polymerase chain reaction (PCR), DNA was extracted and the internal transcribed spacer sequence was used. The amplified products were then subjected to restriction fragment length polymorphism and phylogenetic analyses. Among previously infected patients, 40% (12/30) showed positive results in the rK-39 strip test. The nested PCR positive rates for previously infected patients/relatives and livestock samples were 86% (77/90) and 80% (8/10), respectively. Moreover, the phylogenetic analysis showed that the pathogen was The results of this study provide a basis for developing disease prevention and control strategies for leishmaniasis.
Sections du résumé
Background
Leishmaniasis is a regional infectious disease caused by the bite of
Methods
The rK-39 strip test was performed on blood samples from previously infected patients. Blood samples were collected from the patients and their families. The blood, liver, spleen, and diaphragm muscle samples were collected from livestock. To perform nested polymerase chain reaction (PCR), DNA was extracted and the internal transcribed spacer sequence was used. The amplified products were then subjected to restriction fragment length polymorphism and phylogenetic analyses.
Results
Among previously infected patients, 40% (12/30) showed positive results in the rK-39 strip test. The nested PCR positive rates for previously infected patients/relatives and livestock samples were 86% (77/90) and 80% (8/10), respectively. Moreover, the phylogenetic analysis showed that the pathogen was
Conclusions
The results of this study provide a basis for developing disease prevention and control strategies for leishmaniasis.
Identifiants
pubmed: 33842392
doi: 10.3389/fcimb.2021.645944
pmc: PMC8029648
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
645944Informations de copyright
Copyright © 2021 Han, Chen, Yang, Feng and Wu.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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