The Enigma of Substrate Recognition and Catalytic Efficiency of APE1-Like Enzymes.

DNA repair abasic site apurinic/apyrimidinic endonuclease pre-steady state kinetics target nucleotide recognition

Journal

Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250

Informations de publication

Date de publication:
2021
Historique:
received: 14 10 2020
accepted: 22 02 2021
entrez: 12 4 2021
pubmed: 13 4 2021
medline: 13 4 2021
Statut: epublish

Résumé

Despite significant achievements in the elucidation of the nature of protein-DNA contacts that control the specificity of nucleotide incision repair (NIR) by apurinic/apyrimidinic (AP) endonucleases, the question on how a given nucleotide is accommodated by the active site of the enzyme remains unanswered. Therefore, the main purpose of our study was to compare kinetics of conformational changes of three homologous APE1-like endonucleases (insect

Identifiants

pubmed: 33842455
doi: 10.3389/fcell.2021.617161
pmc: PMC8033172
doi:

Types de publication

Journal Article

Langues

eng

Pagination

617161

Informations de copyright

Copyright © 2021 Davletgildeeva, Ishchenko, Saparbaev, Fedorova and Kuznetsov.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Anastasiia T Davletgildeeva (AT)

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia.

Alexander A Ishchenko (AA)

Group "Mechanisms of DNA Repair and Carcinogenesis", Equipe Labellisée LIGUE 2016, CNRS UMR 9019, Université Paris-Saclay, Villejuif, France.

Murat Saparbaev (M)

Group "Mechanisms of DNA Repair and Carcinogenesis", Equipe Labellisée LIGUE 2016, CNRS UMR 9019, Université Paris-Saclay, Villejuif, France.

Olga S Fedorova (OS)

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Nikita A Kuznetsov (NA)

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Classifications MeSH