Dexmedetomidine exhibits antiarrhythmic effects on human-induced pluripotent stem cell-derived cardiomyocytes through a Na/Ca channel-mediated mechanism.

Dexmedetomidine (DMED) electrophysiology human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) patch clamp recording

Journal

Annals of translational medicine
ISSN: 2305-5839
Titre abrégé: Ann Transl Med
Pays: China
ID NLM: 101617978

Informations de publication

Date de publication:
Mar 2021
Historique:
entrez: 12 4 2021
pubmed: 13 4 2021
medline: 13 4 2021
Statut: ppublish

Résumé

Ventricular-like human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exhibit the electrophysiological characteristics of spontaneous beating. Previous studies demonstrated that dexmedetomidine (DMED), a highly selective and widely used α A conventional patch-clamp recording method was used to investigate the direct effects of DMED on spontaneous action potentials, pacemaker currents ( DMED dose-dependently altered the frequency of ventricular-like spontaneous action potentials with a half-maximal inhibitory concentration (IC DMED could inhibit the frequency of spontaneous action potentials and decrease the

Sections du résumé

BACKGROUND BACKGROUND
Ventricular-like human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exhibit the electrophysiological characteristics of spontaneous beating. Previous studies demonstrated that dexmedetomidine (DMED), a highly selective and widely used α
METHODS METHODS
A conventional patch-clamp recording method was used to investigate the direct effects of DMED on spontaneous action potentials, pacemaker currents (
RESULTS RESULTS
DMED dose-dependently altered the frequency of ventricular-like spontaneous action potentials with a half-maximal inhibitory concentration (IC
CONCLUSIONS CONCLUSIONS
DMED could inhibit the frequency of spontaneous action potentials and decrease the

Identifiants

DOI: 10.21037/atm-20-5898 PMID: 33842620 PMC: PMC8033317
pubmed: 33842620
doi: 10.21037/atm-20-5898
pii: atm-09-05-399
pmc: PMC8033317
doi:

Types de publication

Journal Article

Langues

eng

Pagination

399

Informations de copyright

2021 Annals of Translational Medicine. All rights reserved.

Déclaration de conflit d'intérêts

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/atm-20-5898). The authors have no conflicts of interest to declare.

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Auteurs

Li Yang (L)

Department of Anesthesiology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Yiqi Gong (Y)

Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Yao Tan (Y)

Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Lei Wu (L)

Department of Anesthesiology, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Nevin Witman (N)

Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.

Jijian Zheng (J)

Department of Anesthesiology, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Jun Zhang (J)

Department of Anesthesiology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Wei Fu (W)

Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Wei Wang (W)

Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center; School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Classifications MeSH