Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2.
COVID-19
CRISPR-Cas9
RT-RPA
SARS-CoV-2
VirD2
biosensors
dCas9
lateral flow assay
molecular diagnostics
nucleic acid detection
relaxases
Journal
Nano letters
ISSN: 1530-6992
Titre abrégé: Nano Lett
Pays: United States
ID NLM: 101088070
Informations de publication
Date de publication:
28 04 2021
28 04 2021
Historique:
pubmed:
13
4
2021
medline:
25
6
2021
entrez:
12
4
2021
Statut:
ppublish
Résumé
Rapid, sensitive, and specific point-of-care testing for pathogens is crucial for disease control. Lateral flow assays (LFAs) have been employed for nucleic acid detection, but they have limited sensitivity and specificity. Here, we used a fusion of catalytically inactive SpCas9 endonuclease and VirD2 relaxase for sensitive, specific nucleic acid detection by LFA. In this assay, the target nucleic acid is amplified with biotinylated oligos. VirD2-dCas9 specifically binds the target sequence via dCas9 and covalently binds to a FAM-tagged oligonucleotide via VirD2. The biotin label and FAM tag are detected by a commercially available LFA. We coupled this system, named Vigilant (
Identifiants
pubmed: 33844549
doi: 10.1021/acs.nanolett.1c00612
pmc: PMC8056947
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3596-3603Références
Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1613-8
pubmed: 8643679
Lancet. 2020 Feb 15;395(10223):470-473
pubmed: 31986257
Genes Dev. 2013 Dec 1;27(23):2602-14
pubmed: 24298059
Nat Biotechnol. 2020 Jul;38(7):870-874
pubmed: 32300245
ACS Nano. 2020 Feb 25;14(2):2497-2508
pubmed: 32045522
Cell. 2021 Jan 21;184(2):323-333.e9
pubmed: 33306959
Mol Plant. 2015 Aug;8(8):1288-91
pubmed: 25749112
Front Microbiol. 2020 Dec 17;11:610872
pubmed: 33391239
N Engl J Med. 2020 Oct 8;383(15):1492-1494
pubmed: 32937062
J Bacteriol. 1992 Jan;174(1):303-8
pubmed: 1309520
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
iScience. 2020 Aug 21;23(8):101406
pubmed: 32771976
Eur J Clin Microbiol Infect Dis. 2020 Jun;39(6):1059-1061
pubmed: 32342252
Methods Enzymol. 2014;546:1-20
pubmed: 25398333
Plant Biotechnol J. 2015 May;13(4):578-89
pubmed: 25400128
Science. 2018 Apr 27;360(6387):436-439
pubmed: 29449511
Bull World Health Organ. 2017 Sep 1;95(9):639-645
pubmed: 28867844
Nature. 2020 Jun;582(7811):277-282
pubmed: 32349121
Nat Commun. 2020 Nov 20;11(1):5920
pubmed: 33219228
J Cell Biol. 2016 Aug 29;214(5):529-37
pubmed: 27551060
Science. 2018 Nov 16;362(6416):839-842
pubmed: 30337455
Sci Adv. 2019 Nov 13;5(11):eaaw9807
pubmed: 31763447
Infect Drug Resist. 2019 Jul 30;12:2343-2353
pubmed: 31440069
Nat Protoc. 2019 Oct;14(10):2986-3012
pubmed: 31548639
EMBO Rep. 2004 Jun;5(6):632-7
pubmed: 15153934
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Sci Rep. 2016 Nov 25;6:37732
pubmed: 27886248
Commun Biol. 2020 Jan 23;3(1):44
pubmed: 31974493
Annu Rev Biomed Eng. 2020 Jun 4;22:371-386
pubmed: 32501770
Sci Rep. 2019 Dec 31;9(1):20393
pubmed: 31892706
ACS Synth Biol. 2020 Jun 19;9(6):1226-1233
pubmed: 32159950
Nat Commun. 2020 Nov 20;11(1):5921
pubmed: 33219225
Sci Rep. 2015 Feb 09;5:8345
pubmed: 25662162
Virus Res. 2020 Oct 15;288:198129
pubmed: 32822689
Nat Biotechnol. 2020 Jul;38(7):769-772
pubmed: 32641849
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
World J Microbiol Biotechnol. 2017 Sep 25;33(10):183
pubmed: 28948456
AMB Express. 2018 Sep 29;8(1):155
pubmed: 30269246