Probing bacterial cell wall growth by tracing wall-anchored protein complexes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 04 2021
12 04 2021
Historique:
received:
22
05
2020
accepted:
12
03
2021
entrez:
13
4
2021
pubmed:
14
4
2021
medline:
22
4
2021
Statut:
epublish
Résumé
The dynamic assembly of the cell wall is key to the maintenance of cell shape during bacterial growth. Here, we present a method for the analysis of Escherichia coli cell wall growth at high spatial and temporal resolution, which is achieved by tracing the movement of fluorescently labeled cell wall-anchored flagellar motors. Using this method, we clearly identify the active and inert zones of cell wall growth during bacterial elongation. Within the active zone, the insertion of newly synthesized peptidoglycan occurs homogeneously in the axial direction without twisting of the cell body. Based on the measured parameters, we formulate a Bernoulli shift map model to predict the partitioning of cell wall-anchored proteins following cell division.
Identifiants
pubmed: 33846341
doi: 10.1038/s41467-021-22483-8
pii: 10.1038/s41467-021-22483-8
pmc: PMC8042023
doi:
Substances chimiques
Escherichia coli Proteins
0
Multiprotein Complexes
0
Peptidoglycan
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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