Anti-C1-Inhibitor Autoantibody Detection by ELISA.


Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 13 4 2021
pubmed: 14 4 2021
medline: 23 6 2021
Statut: ppublish

Résumé

Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitor autoantibodies by an indirect ELISA. In this method patients' sera are incubated in a microplate coated with plasma derived C1-inhibitor.

Identifiants

pubmed: 33847936
doi: 10.1007/978-1-0716-1016-9_11
doi:

Substances chimiques

Autoantibodies 0
Complement C1 Inactivator Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

115-120

Références

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Auteurs

Chiara Suffritti (C)

ASST-Fatebenefratelli-Sacco, Milan, Italy. chiarasuffritti@gmail.com.

Sonia Caccia (S)

Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.

Silvia Berra (S)

Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.

Debora Parolin (D)

Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.

Marco Cicardi (M)

ASST-Fatebenefratelli-Sacco, Milan, Italy.
Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.

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