DDB1- and CUL4-associated factor 8 plays a critical role in spermatogenesis.
Dcaf8
male infertility
spermatogenesis
Journal
Frontiers of medicine
ISSN: 2095-0225
Titre abrégé: Front Med
Pays: China
ID NLM: 101549428
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
26
10
2020
accepted:
20
02
2021
pubmed:
16
4
2021
medline:
30
4
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
Cullin-RING E3 ubiquitin ligase (CRL)-4 is a member of the large CRL family in eukaryotes. It plays important roles in a wide range of cellular processes, organismal development, and physiological and pathological conditions. DDB1- and CUL4-associated factor 8 (DCAF8) is a WD40 repeat-containing protein, which serves as a substrate receptor for CRL4. The physiological role of DCAF8 is unknown. In this study, we constructed Dcaf8 knockout mice. Homozygous mice were viable with no noticeable abnormalities. However, the fertility of Dcaf8-deficient male mice was markedly impaired, consistent with the high expression of DCAF8 in adult mouse testis. Sperm movement characteristics, including progressive motility, path velocity, progressive velocity, and track speed, were significantly lower in Dcaf8 knockout mice than in wild-type (WT) mice. However, the total motility was similar between WT and Dcaf8 knockout sperm. More than 40% of spermatids in Dcaf8 knockout mice showed pronounced morphological abnormalities with typical bent head malformation. The acrosome and nucleus of Dcaf8 knockout sperm looked similar to those of WT sperm. In vitro tests showed that the fertilization rate of Dcaf8 knockout mice was significantly reduced. The results demonstrated that DCAF8 plays a critical role in spermatogenesis, and DCAF8 is a key component of CRL4 function in the reproductive system.
Identifiants
pubmed: 33855678
doi: 10.1007/s11684-021-0851-8
pii: 10.1007/s11684-021-0851-8
doi:
Substances chimiques
Cullin Proteins
0
DNA-Binding Proteins
0
Ddb1 protein, mouse
0
Factor VIII
9001-27-8
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
302-312Références
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