New pectic polysaccharides from Codonopsis pilosula and Codonopsis tangshen: structural characterization and cellular antioxidant activities.
Codonopsis pilosula
Codonopsis tangshen
antioxidation
pectic polysaccharides
structural features
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
17
03
2021
received:
07
11
2020
accepted:
15
04
2021
pubmed:
16
4
2021
medline:
21
10
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
Codonopsis pilosula and Codonopsis tangshen are plants widely used in traditional Chinese medicine. Two pectic polysaccharides from the roots of C. pilosula and C. tangshen named as CPP-1 and CTP-1 were obtained by boiling water extraction and column chromatography. The core structures of both CPP-1 and CTP-1 comprise the long homogalacturonan region (HG) as the backbone and the rhamnogalacturonan I (RG-I) region as the side chains. CPP-1 has methyl esterified galacturonic acid units and a slightly lower molecular weight than CTP-1. Biological testing suggested that CPP-1 and CTP-1 can protect IPEC-J2 cells against the H All of the results indicated that pectic polysaccharides CPP-1 and CTP-1 from different species of C. pilosula roots could be used as a potential natural antioxidant source. These findings will be valuable for further studies and new applications of pectin-containing health products. © 2021 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Codonopsis pilosula and Codonopsis tangshen are plants widely used in traditional Chinese medicine. Two pectic polysaccharides from the roots of C. pilosula and C. tangshen named as CPP-1 and CTP-1 were obtained by boiling water extraction and column chromatography.
RESULTS
RESULTS
The core structures of both CPP-1 and CTP-1 comprise the long homogalacturonan region (HG) as the backbone and the rhamnogalacturonan I (RG-I) region as the side chains. CPP-1 has methyl esterified galacturonic acid units and a slightly lower molecular weight than CTP-1. Biological testing suggested that CPP-1 and CTP-1 can protect IPEC-J2 cells against the H
CONCLUSION
CONCLUSIONS
All of the results indicated that pectic polysaccharides CPP-1 and CTP-1 from different species of C. pilosula roots could be used as a potential natural antioxidant source. These findings will be valuable for further studies and new applications of pectin-containing health products. © 2021 Society of Chemical Industry.
Substances chimiques
Antioxidants
0
NF-E2 Transcription Factor
0
Plant Extracts
0
Pectins
89NA02M4RX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6043-6052Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2016M602704
Organisme : International Cooperation Projects of Science & Technology Department of Sichuan Province
ID : 2017HH0093
Organisme : Research Council of Norway
ID : 226244/F50
Organisme : Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System
ID : SCCXTD-2020-18
Informations de copyright
© 2021 Society of Chemical Industry.
Références
Aw TY, Molecular and cellular responses to oxidative stress and changes in oxidation-reduction imbalance in the intestine. Am J Clin Nutr 70:557-565 (1999).
Luo D, Identification of structure and antioxidant activity of a fraction of polysaccharide purified from Dioscorea nipponica Makino. Carbohydr Polym 71:544-549 (2008).
Schieber M and Chandel NS, ROS function in redox signaling and oxidative stress. Curr Biol 24:R453-R462 (2014).
Brieger K, Schiavone S, Miller FJ and Krause KH, Reactive oxygen species: from health to disease. Swiss Med Wkly 142:1-14 (2012).
D'Autréaux B and Toledano MB, ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nat Rev Mol Cell Biol 8:813-824 (2007).
Ma N, Guo P, Zhang J, He T, Kim SW, Zhang G et al., Nutrients mediate intestinal bacteria-mucosal immune crosstalk. Front Immunol 9:5 (2018).
Bhattacharyya A, Chattopadhyay R, Mitra S and Crowe SE, Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev 94:329-354 (2014).
Gao SM, Liu JS, Wang M, Cao TT, Qi YD, Zhang BG et al., Traditional uses, phytochemistry, pharmacology and toxicology of Codonopsis: a review. J Ethnopharmacol 219:50-70 (2018).
He Q, Zhu E, Wang Z, Chou G, Xu L and Hu Z, Study on chemical constitutes of Codonopsis pilosula. Chinese Pharm J 1: 10-12 (2006).
Kim EY, Kim JA, Jeon HJ, Kim S, Kim YH, Kim HY et al., Chemical fingerprinting of Codonopsis pilosula and simultaneous analysis of its major components by HPLC-UV. Arch Pharm Res 37:1148-1158 (2014).
Lin L-C, Tsai T-H and Kuo C-L, Chemical constituents comparison of Codonopsis tangshen Codonopsis pilosula var. modesta and Codonopsis pilosula. Nat Prod Res 27:1812-1815 (2013).
Feng HL and Gao JP, Study of polysaccharides in Codonopsis pilosula on antineoplastic activity in vivo and the acute toxicity testing. Shanxi J Tradit Chinese Med 28:49-50 (2012).
Qin T, Ren Z, Liu X, Luo Y, Long Y, Peng S et al., Study of the selenizing Codonopsis pilosula polysaccharides protects RAW264.7 cells from hydrogen peroxide-induced injury. Int J Biol Macromol 125:534-543 (2019).
Li QY, Zhu QF, Liu CL, Wei X and Zhu RR, Isolation and purification of Codonopsis pilosula polysaccharide and its anti-oxidant activity. Chin Tradit Herb Drugs 48:907-912 (2017).
Zou Y, Zhang Y, Smestad B, Rise F, Chen Z, Jia R et al., Structural features of pectic polysaccharides from stems of two species of radix Codonopsis and their antioxidant activities. Int J Biol Macromol 159:704-713 (2020).
Singleton V and Rossi J, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144-158 (1964).
Bradford MM, Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of thymus daenensis Celak. Anal Biochem 72:248-254 (1976).
Dubois M, Gilles KA, Hamilton JK, Rebers PA and Smith F, Colorimetric method for determination of sugars and related substances. Anal Chem 28:350-356 (1956).
Sun QL, Li YX, Cui YS, Jiang SL, Dong CX and Du J, Structural characterization of three polysaccharides from the roots of Codonopsis pilosula and their immunomodulatory effects on RAW264.7 macrophages. Int J Biol Macromol 130:556-563 (2019).
Han F-M, Cheng L-L and Chen Y, Study on isolation and composition of Codonopsis tangshen polysaccharides. Chin Pharm J-Beijing 40:1381-1383 (2005).
Bai R, Li W, Li Y, Ma M, Wang Y, Zhang J et al., Cytotoxicity of two water-soluble polysaccharides from Codonopsis pilosula Nannf. var. modesta (Nannf.) L.T.Shen against human hepatocellular carcinoma HepG2 cells and its mechanism. Int J Biol Macromol 120:1544-1550 (2018).
Yang C, Gou Y, Chen J, An J, Chen W and Hu F, Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula. Carbohydr Polym 98:886-895 (2013).
Zou YF, Chen XF, Malterud KE, Rise F, Barsett H, Inngjerdingen KT et al., Structural features and complement fixing activity of polysaccharides from Codonopsis pilosula Nannf. Var. modesta L.T.Shen roots. Carbohydr Polym 113:420-429 (2014).
Zou YF, Zhang YY, Fu YP, Inngjerdingen KT, Paulsen BS, Feng B et al., A polysaccharide isolated from Codonopsis pilosula with immunomodulation effects both in vitro and in vivo. Molecules 24:1-13 (2019).
Waldron KW and Faulds CB, Cell Wall polysaccharides: composition and structure. Compr Glycosci From Chem to Syst Biol 1-4:181-201 (2007).
Maxwell EG, Belshaw NJ, Waldron KW and Morris VJ, Pectin - An emerging new bioactive food polysaccharide. Trends Food Sci Technol 24:64-73 (2012).
Zhang P, Hu L, Bai R, Zheng X, Ma Y, Gao X et al., Structural characterization of a pectic polysaccharide from Codonopsis pilosula and its immunomodulatory activities in vivo and in vitro. Int J Biol Macromol 104:1359-1369 (2017).
Habibi Y, Heyraud A, Mahrouz M and Vignon MR, Structural features of pectic polysaccharides from the skin of Opuntia ficus-indica prickly pear fruits. Carbohydr Res 339:1119-1127 (2004).
Hromádková Z, Košťálová Z, Vrchotová N and Ebringerová A, Non-cellulosic polysaccharides from the leaves of small balsam (Impatiens parviflora DC.). Carbohydr Res 389:147-153 (2014).
Kostalova Z, Hromádková Z and Ebringerová A, Structural diversity of pectins isolated from the Styrian oil-pumpkin (Cucurbita pepo var. styriaca) fruit. Carbohydr Polym 93:163-171 (2013).
Chen J, Pang W, Kan Y, Zhao L, He Z, Shi W et al., Structure of a pectic polysaccharide from Pseudostellaria heterophylla and stimulating insulin secretion of INS-1 cell and distributing in rats by oral. Int J Biol Macromol 106:456-463 (2018).
Wen ZS, Ma L, Xiang XW, Tang Z, Guan RF and Le Qu Y, Protective effect of low molecular-weight seleno-aminopolysaccharides against H2O2-induecd oxidative stress in intestinal epithelial cells. Int J Biol Macromol 112:745-753 (2018).
Circu ML and Aw TY, Intestinal redox biology and oxidative stress. Semin Cell Dev Biol 23:729-737 (2012).
He L, Zhang J, Zhao J, Ma N, Kim SW, Qiao S et al., Autophagy: the last defense against cellular nutritional stress. Adv Nutr 9:493-504 (2018).
Del Rio D, Stewart AJ and Pellegrini N, A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 15:316-328 (2005).
Wang Z-Y, Loo TY, Shen J-G, Wang N, Wang D-M, Yang D-P et al., LDH-A silencing suppresses breast cancer tumorigenicity through induction of oxidative stress mediated mitochondrial pathway apoptosis. Breast Cancer Res Treat 131:791-800 (2012).
Magesh S, Yu C and Hu L, Small molecule modulators of Keap1-Nrf2-ARE pathway as potential preventive and therapeutic agents. Med Res Rev 32:687-726 (2012).
Nguyen T, Nioi P and Pickett CB, The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress. J Biol Chem 284:13291-13295 (2009).
Tang X, Liu B, Wang X, Yu Q and Fang R, Epidermal growth factor, through alleviating oxidative stress, protect IPEC-J2 cells from lipopolysaccharides-induced apoptosis. Int J Mol Sci 19:1-15 (2018).
Ma J, He W-L, Gao C-Y, Yu R-Y, Xue P and Niu Y-C, Antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides on hypoxic- ischemic brain injury induced by Nrf2 pathway. Chin J Clin Anat 37:403-408 (2019).
Song D, Cheng Y, Li X, Wang F, Lu Z, Xiao X et al., Biogenic Nanoselenium particles effectively attenuate oxidative stress-induced intestinal epithelial barrier injury by activating the Nrf2 antioxidant pathway. ACS Appl Mater Interfaces 9:14724-14740 (2017).
Mukaigasa K, Tsujita T, Nguyen VT, Li L, Yagi H, Fuse Y et al., Nrf2 activation attenuates genetic endoplasmic reticulum stress induced by a mutation in the phosphomannomutase 2 gene in zebrafish. Proc Natl Acad Sci U S A 115:2758-2763 (2018).
Yang F, Wang A, Zeng X, Hou C, Liu H and Qiao S, Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions. BMC Microbiol 15:1-11 (2015).
Zhang Z, Wang X, Mo X and Qi H, Degradation and the antioxidant activity of polysaccharide from Enteromorpha linza. Carbohydr Polym 92:2084-2087 (2013).
Lo TCT, Chang CA, Chiu KH, Tsay PK and Jen JF, Correlation evaluation of antioxidant properties on the monosaccharide components and glycosyl linkages of polysaccharide with different measuring methods. Carbohydr Polym 86:320-327 (2011).
Zhang L, Hu Y, Duan X, Tang T, Shen Y, Hu B et al., Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms. Int J Biol Macromol 113:1-7 (2018).
Huang C, Cao X, Chen X, Fu Y, Zhu Y, Chen Z et al., A pectic polysaccharide from Ligusticum chuanxiong promotes intestine antioxidant defense in aged mice. Carbohydr Polym 174:915-922 (2017).
Yao R, Huang C, Chen X, Yin Z, Fu Y, Li L et al., Two complement fixing pectic polysaccharides from pedicel of Lycium barbarum L. promote cellular antioxidant defense. Int J Biol Macromol 112:356-363 (2018).
Zou YF, Fu YP, Chen XF, Austarheim I, Inngjerdingen KT, Huang C et al., Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong. Molecules 22:1-11 (2017).
Zhu R, Zhang X, Wang Y, Zhang L, Zhao J, Chen G et al., Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities. Carbohydr Polym 210:73-84 (2019).
Liu C, Chen J, Li E, Fan Q, Wang D, Li P et al., The comparison of antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide (CP) and sulfated CP. Int Immunopharmacol 24:299-305 (2015).