Homeodomain proteins POU-M2, antennapedia and abdominal-B are involved in regulation of the segment-specific expression of the clip-domain serine protease gene CLIP13 in the silkworm, Bombyx mori.
20-hydroxyecdysone
Bombyx mori
POU-M2
clip-domain serine protease
epidermis
homeotic gene
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
20
02
2021
received:
07
11
2020
accepted:
26
02
2021
pubmed:
17
4
2021
medline:
19
2
2022
entrez:
16
4
2021
Statut:
ppublish
Résumé
Clip-domain serine proteases (CLIPs) play important roles in insect innate immunity and development. Our previous studies indicated that CLIP13, an epidermis-specific gene, was involved in cuticle remodeling during molting and metamorphosis in the silkworm, Bombyx mori. However, the transcriptional regulatory mechanism and regulatory pathways of CLIP13 remained unclear. In the present study, we investigated CLIP13 expression and the regulation pathway controlled by 20-hydroxyecdysone (20E) in the silkworm. At the transcriptional level, expression of CLIP13 exhibited pronounced spatial and temporal specificity in different regions of the epidermis; homeodomain transcription factors POU-M2, antennapedia (Antp), and abdominal-B (Abd-B) showed similar expression change trends as CLIP13 in the head capsule, thorax, and abdomen, respectively. Furthermore, results of cell transfection assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation demonstrated that POU-M2, Antp, and Abd-B were involved in the transcriptional regulation of CLIP13 by directly binding to their cis-response elements in CLIP13 promoter. RNA interference-mediated silencing of POU-M2, Antp, and Abd-B led to a decrease of CLIP13 expression in the head capsule, the epidermis of the 1st to 3rd thoracic segments and the 7th to 10th abdominal segments, respectively. Consistent with CLIP13, 20E treatment significantly upregulated expression of POU-M2, Antp, and Abd-B in the silkworm epidermis. Taken together, these data suggest that 20E positively regulates transcription of CLIP13 via homeodomain proteins POU-M2, Antp, and Abd-B in different regions of the silkworm epidermis during metamorphosis, thus affecting the molting process. Our findings provide new insight into the functions of homeodomain transcription factors in insect molting.
Identifiants
pubmed: 33860633
doi: 10.1111/1744-7917.12916
doi:
Substances chimiques
Homeodomain Proteins
0
Insect Proteins
0
Serine
452VLY9402
Ecdysterone
5289-74-7
Serine Proteases
EC 3.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111-127Subventions
Organisme : National Natural Science Foundation of China
ID : 31772532
Organisme : National Natural Science Foundation of China
ID : 31702187
Organisme : China Postdoctoral Science Foundation
ID : 2019M663880XB
Organisme : Natural Science Foundation of Chongqing, China
ID : cstc2019jcyj-bshX0033
Informations de copyright
© 2021 Institute of Zoology, Chinese Academy of Sciences.
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