Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome.
Basal Bodies
/ physiology
Casein Kinase I
/ antagonists & inhibitors
Cytokinesis
/ physiology
Cytoplasm
/ metabolism
DNA Cleavage
DNA Replication
DNA, Kinetoplast
/ metabolism
Mitochondria
/ metabolism
Protozoan Proteins
/ metabolism
RNA Interference
RNA, Small Interfering
/ genetics
Signal Transduction
Trypanosoma brucei brucei
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
12
2020
accepted:
26
03
2021
entrez:
16
4
2021
pubmed:
17
4
2021
medline:
29
9
2021
Statut:
epublish
Résumé
The single mitochondrial nucleoid (kinetoplast) of Trypanosoma brucei is found proximal to a basal body (mature (mBB)/probasal body (pBB) pair). Kinetoplast inheritance requires synthesis of, and scission of kinetoplast DNA (kDNA) generating two kinetoplasts that segregate with basal bodies into daughter cells. Molecular details of kinetoplast scission and the extent to which basal body separation influences the process are unavailable. To address this topic, we followed basal body movements in bloodstream trypanosomes following depletion of protein kinase TbCK1.2 which promotes kinetoplast division. In control cells we found that pBBs are positioned 0.4 um from mBBs in G1, and they mature after separating from mBBs by at least 0.8 um: mBB separation reaches ~2.2 um. These data indicate that current models of basal body biogenesis in which pBBs mature in close proximity to mBBs may need to be revisited. Knockdown of TbCK1.2 produced trypanosomes containing one kinetoplast and two nuclei (1K2N), increased the percentage of cells with uncleaved kDNA 400%, decreased mBB spacing by 15%, and inhibited cytokinesis 300%. We conclude that (a) separation of mBBs beyond a threshold of 1.8 um correlates with division of kDNA, and (b) TbCK1.2 regulates kDNA scission. We propose a Kinetoplast Division Factor hypothesis that integrates these data into a pathway for biogenesis of two daughter mitochondrial nucleoids.
Identifiants
pubmed: 33861760
doi: 10.1371/journal.pone.0249908
pii: PONE-D-20-39205
pmc: PMC8051774
doi:
Substances chimiques
DNA, Kinetoplast
0
Protozoan Proteins
0
RNA, Small Interfering
0
Casein Kinase I
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0249908Subventions
Organisme : NIAID NIH HHS
ID : R01 AI124046
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI126311
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared no competing interests exist.
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