Insight into the drug resistance whole genome of Mycobacterium tuberculosis isolates from Khyber Pakhtunkhwa, Pakistan.

Drug Resistance, Multiple, Bacterial / genetics Genome, Bacterial / genetics Humans Microbial Sensitivity Tests / methods Mutation / genetics Mycobacterium tuberculosis / drug effects Pakistan Tuberculosis, Multidrug-Resistant / drug therapy Whole Genome Sequencing / methods
Genome PE PPE PZA Resistance

Journal

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138

Informations de publication

Date de publication:
08 2021
Historique:
received: 27 10 2020
revised: 31 03 2021
accepted: 08 04 2021
pubmed: 17 4 2021
medline: 13 1 2022
entrez: 16 4 2021
Statut: ppublish

Résumé

Whole genome sequencing (WGS) is one of the most reliable methods for detection of drug resistance, genetic diversity in other virulence factor and also evolutionary dynamics of Mycobacterium tuberculosis complex (MTBC). First-line anti-tuberculosis drugs are the major weapons against Mycobacterium tuberculosis (MTB). However, the emergence of drug resistance remained a major obstacle towards global tuberculosis (TB) control program 2030, especially in high burden countries including Pakistan. To overcome the resistance and design potent drugs, genomic variations in drugs targets as well as in the virulence and evolutionary factors might be useful for better understanding and designing potential inhibitors. Here we aimed to find genomic variations in the first-line drugs targets, along with other virulence and evolutionary factors among the circulating isolates in Khyber Pakhtunkhwa, Pakistan. Samples were collected and drug susceptibility testing (DST) was performed as per WHO standard. The resistance samples were subjected to WGS. Among the five whole genome sequences, three samples (NCBI BioProject Accession: PRJNA629298, PRJNA629388) harbored 1997, 1162, and 2053 mutations. Some novel mutations have been detected in drugs targets. Similarly, numerous novel variants have also been detected in virulency and evolutionary factors, PE, PPE, and secretory system of MTB isolates. Exploring the genomic variations among the circulating isolates in geographical specific locations might be useful for future drug designing. To the best of our knowledge, this is the first study that provides useful data regarding the insight genomic variations in virulency, evolutionary factors including ESX and PE/PPE as well as drug targets, for better understanding and management of TB in a WHO declared high burden country.

Identifiants

DOI: 10.1016/j.meegid.2021.104861 PMID: 33862292
pubmed: 33862292
pii: S1567-1348(21)00158-1
doi: 10.1016/j.meegid.2021.104861
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104861

Informations de copyright

Copyright © 2021. Published by Elsevier B.V.

Auteurs

Muhammad Tahir Khan (MT)

Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.

Sajid Ali (S)

Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address: sajidjan@live.com.

Anwar Sheed Khan (AS)

Department of Microbiology, Kohat University of Science and Technology and Provincial Tuberculosis Reference Laboratory, Peshawar, Pakistan. Electronic address: anwar786kp@hotmail.cm.

Arif Ali (A)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: arifalii@sjtu.edu.cn.

Abbas Khan (A)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: Abbaskhan@sjtu.edu.cn.

Aman Chandra Kaushik (AC)

Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Muhammad Irfan (M)

Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA. Electronic address: irfanmuhammad@ufl.edu.

Sathishkumar Chinnasamy (S)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China.

Shulin Zhang (S)

Department of Immunology and Microbiology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China. Electronic address: shulinzhang@sjtu.edu.cn.

Yu-Juan Zhang (YJ)

College of Life Sciences, Chongqing Normal University, Chongqing, China. Electronic address: zhangyj@cqnu.edu.cn.

Zhilei Cui (Z)

Zhilei Cui, Department of Respiratory Medicine, XinHua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Amie Jinghua Wei (AJ)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China.

Yanjie Wang (Y)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China.

Mingzhu Zhao (M)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: mingzhuzhao@sjtu.edu.cn.

Kejia Liu (K)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: kejialiu@umail.ucsb.edu.

Heng Wang (H)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: wangheng0802@sjtu.edu.cn.

Muhammad Tariq Zeb (MT)

Khyber Medical University and Senior Research Officer, In-charge Genomic Laboratory, Veterinary Research Institute, Peshawar 25000, Pakistan.

Dong Qing Wei (DQ)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China; Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: dqwei@sjtu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Multirésistance aux médicaments › Multirésistance bactérienne aux médicaments › Insight into the drug resistance whole genome...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Insight into the drug resistance whole genome...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Insight into the drug resistance whole genome...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Insight into the drug resistance whole genome...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Insight into the drug resistance whole genome...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis › Insight into the drug resistance whole genome...
questionsmedicales.fr › Régions géographiques › Asie › Asie de l'Ouest › Asie du Sud › Pakistan › Insight into the drug resistance whole genome...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à Actinomycetales › Infections à Mycobacterium › Tuberculose › Tuberculose multirésistante › Insight into the drug resistance whole genome...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Séquençage du génome entier › Insight into the drug resistance whole genome...