Long-term detection of clodronate in equine plasma by liquid chromatography-tandem mass spectrometry.
LC-MS/MS
SPE on 96-well plate
TMOA methylation
clodronic acid
horse doping control
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
13
04
2021
received:
26
01
2021
accepted:
14
04
2021
pubmed:
20
4
2021
medline:
13
1
2022
entrez:
19
4
2021
Statut:
ppublish
Résumé
Clodronate is a non-nitrogen-containing bisphosphonate drug approved in equine veterinary medicine. Clodronate is prohibited for use in competition horses; therefore, to set up an appropriate control, detection times and screening limits are required. The quantitative method in plasma consisted of addition of chloromethylene diphosphonic acid as internal standard. Automated sample preparation comprised a solid phase extraction with weak anion exchange properties on microplate. After methylation of the residue with trimethyl orthoacetate, analysis was conducted by high-performance liquid chromatography-tandem mass spectrometry. Using a weighting factor of 1/(concentration)
Substances chimiques
Analgesics, Non-Narcotic
0
Clodronic Acid
0813BZ6866
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1527-1534Subventions
Organisme : Dechra Veterinary Products
Informations de copyright
© 2021 John Wiley & Sons, Ltd.
Références
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