Molecular characterization and RNA interference responses of the lethal giant larvae gene in Diabrotica virgifera virgifera adults.
European corn borer
RNA interference
lethal giant larvae
target gene selection
western corn rootworm
Journal
Archives of insect biochemistry and physiology
ISSN: 1520-6327
Titre abrégé: Arch Insect Biochem Physiol
Pays: United States
ID NLM: 8501752
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
15
03
2021
received:
05
01
2021
accepted:
17
03
2021
pubmed:
20
4
2021
medline:
4
6
2021
entrez:
19
4
2021
Statut:
ppublish
Résumé
High specificity for silencing target genes and single-copy target genes that yield clear phenotypes are two important factors for the success of RNA interference (RNAi). The lethal giant larvae (Lgl) gene appears to be an ideal gene for RNAi because RNAi can effectively suppress its expression and results in molting defects and mortality in Tribolium castaneum. To investigate the suitability of this gene for RNAi in other insects, we identified and characterized DvLgl from the western corn rootworm, Diabrotica virgifera virgifera, a species exhibiting high RNAi efficiency. DvLgl was expressed in all developmental stages and tissues investigated. The deduced DvLgl protein showed high amino-acid sequence identities and similar domain architecture to Lgls from other insect species. Despite many similarities among insect Lgls, RNAi-mediated suppression of DvLgl failed to produce a phenotype in D. v. virgifera adults. The difference in developing phenotypes could be attributed greatly to the level of gene suppression and the insect developmental stages for RNAi. These results highlight the variability in RNAi response among insects and showcase the importance of screening multiple target genes when conducting RNAi studies. Our findings are expected to help the design of future RNAi studies and future investigations of Lgl in insects.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21787Subventions
Organisme : U.S. Department of Agriculture
ID : 2014-67013-21714
Informations de copyright
© 2021 Wiley Periodicals LLC.
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