NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF from Clostridium thermocellum.

Clostridium thermocellum
Cell surface Cellulosome NMR assignments Protein module Scaffoldin

Journal

Biomolecular NMR assignments
ISSN: 1874-270X
Titre abrégé: Biomol NMR Assign
Pays: Netherlands
ID NLM: 101472371

Informations de publication

Date de publication:
10 2021
Historique:
received: 24 02 2021
accepted: 10 04 2021
pubmed: 21 4 2021
medline: 5 2 2022
entrez: 20 4 2021
Statut: ppublish

Résumé

The cellulosome is a highly efficient cellulolytic complex containing cellulolytic enzymes and non-catalytic subunits, i.e. scaffoldins, which are assembled by the interactions between the dockerin modules of the enzymes and the cohesin modules of the primary scaffoldins. The cellulosome attaches to the cell surface via the S-layer homology (SLH) modules of the anchoring scaffoldins. Clostridium thermocellum DSM1313 is a thermophilic cellulosome-producing bacterium with great potential in lignocellulose bioconversion and biofuel production. The bacterium contains four anchoring scaffoldins ScaB, ScaC, ScaD and ScaF, among which ScaF is the only one that contains an additional module of unknown function (ScaF-X) between the cohesin and SLH modules. The gene of ScaF is located outside the scaffoldin gene cluster of scaA, scaB, scaC and scaD. Previous studies showed unique regulation properties and function of ScaF compared to other anchoring scaffoldins, which might be related to the additional ScaF-X module. Here we report the NMR chemical shift assignments of ScaF-X from C. thermocellum DSM1313. The well-dispersed NMR spectrum and the secondary structure prediction based on the chemical shifts of ScaF-X indicated that ScaF-X is a well-folded protein module. The chemical shift assignments provide the basis for future studies on the structure of this module and its function in cellulosomes.

Identifiants

DOI: 10.1007/s12104-021-10025-8 PMID: 33876380
pubmed: 33876380
doi: 10.1007/s12104-021-10025-8
pii: 10.1007/s12104-021-10025-8
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

329-334

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Jie Li (J)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Energy Institute, Qingdao, 266101, Shandong, China.
New Energy Shandong Laboratory, Qingdao, 266101, Shandong, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.

Chao Chen (C)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Energy Institute, Qingdao, 266101, Shandong, China.
New Energy Shandong Laboratory, Qingdao, 266101, Shandong, China.

Ya-Jun Liu (YJ)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Energy Institute, Qingdao, 266101, Shandong, China.
New Energy Shandong Laboratory, Qingdao, 266101, Shandong, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.

Qiu Cui (Q)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China.
Shandong Energy Institute, Qingdao, 266101, Shandong, China.
New Energy Shandong Laboratory, Qingdao, 266101, Shandong, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.

Edward A Bayer (EA)

Department of Biomolecular Sciences, The Weizmann Institute of Science, 7610001, Rehovot, Israel.
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, 8499000, Beer-Sheva, Israel.

Yingang Feng (Y)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China. fengyg@qibebt.ac.cn.
Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China. fengyg@qibebt.ac.cn.
Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China. fengyg@qibebt.ac.cn.
Shandong Energy Institute, Qingdao, 266101, Shandong, China. fengyg@qibebt.ac.cn.
New Energy Shandong Laboratory, Qingdao, 266101, Shandong, China. fengyg@qibebt.ac.cn.
University of Chinese Academy of Sciences, Beijing, 100049, China. fengyg@qibebt.ac.cn.
ORCID: 0000-0002-0879-1316

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Clostridium thermocellum › NMR chemical shift assignments of a module of...