Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes.
3T3-L1 Cells
Adipocytes
/ metabolism
Adiponectin
/ genetics
Animals
Cytokines
/ genetics
Gene Expression
Glycine
/ pharmacology
Mice
NF-kappa B
/ antagonists & inhibitors
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ genetics
Receptors, Glycine
/ genetics
Receptors, Tumor Necrosis Factor, Type I
/ antagonists & inhibitors
Receptors, Tumor Necrosis Factor, Type II
/ antagonists & inhibitors
Adipokines
Glycine receptor
Inflammation
TNF-α receptor
siRNA
Journal
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN: 1420-908X
Titre abrégé: Inflamm Res
Pays: Switzerland
ID NLM: 9508160
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
03
12
2020
accepted:
05
04
2021
revised:
16
02
2021
pubmed:
21
4
2021
medline:
11
11
2021
entrez:
20
4
2021
Statut:
ppublish
Résumé
To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells. RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA. Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor. These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.
Identifiants
pubmed: 33877377
doi: 10.1007/s00011-021-01462-1
pii: 10.1007/s00011-021-01462-1
doi:
Substances chimiques
Adiponectin
0
Adipoq protein, mouse
0
Cytokines
0
NF-kappa B
0
RNA, Messenger
0
RNA, Small Interfering
0
Receptors, Glycine
0
Receptors, Tumor Necrosis Factor, Type I
0
Receptors, Tumor Necrosis Factor, Type II
0
Tnfrsf1a protein, mouse
0
Tnfrsf1b protein, mouse
0
Glycine
TE7660XO1C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
605-618Références
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