Influence of calcium ion-modified implant surfaces in protein adsorption and implant integration.
Blood coagulation
Implant surface design
Osseointegration
Protein adsorption
Titanium implants
Journal
International journal of implant dentistry
ISSN: 2198-4034
Titre abrégé: Int J Implant Dent
Pays: Germany
ID NLM: 101676532
Informations de publication
Date de publication:
21 04 2021
21 04 2021
Historique:
received:
18
11
2020
accepted:
01
03
2021
entrez:
21
4
2021
pubmed:
22
4
2021
medline:
25
11
2021
Statut:
epublish
Résumé
Calcium (Ca) is a well-known element in bone metabolism and blood coagulation. Here, we investigate the link between the protein adsorption pattern and the in vivo responses of surfaces modified with calcium ions (Ca-ion) as compared to standard titanium implant surfaces (control). We used LC-MS/MS to identify the proteins adhered to the surfaces after incubation with human serum and performed bilateral surgeries in the medial section of the femoral condyles of 18 New Zealand white rabbits to test osseointegration at 2 and 8 weeks post-implantation (n=9). Ca-ion surfaces adsorbed 181.42 times more FA10 and 3.85 times less FA12 (p<0.001), which are factors of the common and the intrinsic coagulation pathways respectively. We also detected differences in A1AT, PLMN, FA12, KNG1, HEP2, LYSC, PIP, SAMP, VTNC, SAA4, and CFAH (p<0.01). At 2 and 8 weeks post-implantation, the mean bone implant contact (BIC) with Ca-ion surfaces was respectively 1.52 and 1.25 times higher, and the mean bone volume density (BVD) was respectively 1.35 and 1.13 times higher. Differences were statistically significant for BIC at 2 and 8 weeks and for BVD at 2 weeks (p<0.05). The strong thrombogenic protein adsorption pattern at Ca-ion surfaces correlated with significantly higher levels of implant osseointegration. More effective implant surfaces combined with smaller implants enable less invasive surgeries, shorter healing times, and overall lower intervention costs, especially in cases of low quantity or quality of bone.
Sections du résumé
BACKGROUND
Calcium (Ca) is a well-known element in bone metabolism and blood coagulation. Here, we investigate the link between the protein adsorption pattern and the in vivo responses of surfaces modified with calcium ions (Ca-ion) as compared to standard titanium implant surfaces (control). We used LC-MS/MS to identify the proteins adhered to the surfaces after incubation with human serum and performed bilateral surgeries in the medial section of the femoral condyles of 18 New Zealand white rabbits to test osseointegration at 2 and 8 weeks post-implantation (n=9).
RESULTS
Ca-ion surfaces adsorbed 181.42 times more FA10 and 3.85 times less FA12 (p<0.001), which are factors of the common and the intrinsic coagulation pathways respectively. We also detected differences in A1AT, PLMN, FA12, KNG1, HEP2, LYSC, PIP, SAMP, VTNC, SAA4, and CFAH (p<0.01). At 2 and 8 weeks post-implantation, the mean bone implant contact (BIC) with Ca-ion surfaces was respectively 1.52 and 1.25 times higher, and the mean bone volume density (BVD) was respectively 1.35 and 1.13 times higher. Differences were statistically significant for BIC at 2 and 8 weeks and for BVD at 2 weeks (p<0.05).
CONCLUSIONS
The strong thrombogenic protein adsorption pattern at Ca-ion surfaces correlated with significantly higher levels of implant osseointegration. More effective implant surfaces combined with smaller implants enable less invasive surgeries, shorter healing times, and overall lower intervention costs, especially in cases of low quantity or quality of bone.
Identifiants
pubmed: 33880662
doi: 10.1186/s40729-021-00314-1
pii: 10.1186/s40729-021-00314-1
pmc: PMC8058122
doi:
Substances chimiques
Ions
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
32Références
J Biomed Mater Res A. 2015 Aug;103(8):2661-72
pubmed: 25631679
Platelets. 2004 Feb;15(1):29-35
pubmed: 14985174
J Periodontol. 2013 Aug;84(8):1180-90
pubmed: 23088531
Int Immunopharmacol. 2008 Feb;8(2):135-42
pubmed: 18182216
Biomaterials. 1994 Aug;15(10):827-34
pubmed: 7986948
J Biomed Mater Res B Appl Biomater. 2018 Jan;106(1):421-432
pubmed: 28186691
Biomaterials. 2011 Apr;32(11):2757-74
pubmed: 21292319
BioDrugs. 2013 Apr;27(2):97-111
pubmed: 23329397
J Biomed Mater Res A. 2013 May;101(5):1478-88
pubmed: 23135872
Biophys Chem. 2004 Dec 20;112(2-3):117-30
pubmed: 15572239
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Tissue Eng Part B Rev. 2014 Dec;20(6):697-712
pubmed: 24906469
Biofouling. 2017 Jan;33(1):98-111
pubmed: 28005415
Colloids Surf B Biointerfaces. 2015 Jun 1;130:173-81
pubmed: 25886795
Biomed Mater Eng. 1998;8(1):1-9
pubmed: 9713681
J R Soc Interface. 2010 Jan 6;7(42):81-90
pubmed: 19369221
J Hepatol. 2016 Jun;64(6):1416-27
pubmed: 26921689
J Biomed Mater Res A. 2015 Mar;103(3):969-80
pubmed: 24862163
Tissue Eng Part A. 2010 Aug;16(8):2467-73
pubmed: 20214455
Drug Discov Today. 2018 Apr;23(4):879-890
pubmed: 29407177
J Nanobiotechnology. 2008 Feb 19;6:3
pubmed: 18284677
Colloids Surf B Biointerfaces. 2013 Mar 1;103:395-404
pubmed: 23261559
J Biol Inorg Chem. 2018 May;23(3):459-470
pubmed: 29572572
Cancer Lett. 2014 Apr 1;345(1):27-38
pubmed: 24333733
Clin Oral Implants Res. 2006 Oct;17 Suppl 2:55-67
pubmed: 16968382
Colloids Surf B Biointerfaces. 2019 Sep 1;181:125-133
pubmed: 31128512
Langmuir. 2013 Jan 22;29(3):902-12
pubmed: 23095019
Skeletal Radiol. 2006 Jan;35(1):34-41
pubmed: 16247642
Biofouling. 2017 Sep;33(8):676-689
pubmed: 28871865
Thromb Haemost. 1999 Jul;82(1):58-64
pubmed: 10456455
Indian J Anaesth. 2014 Sep;58(5):515-23
pubmed: 25535411
Biomaterials. 2005 Apr;26(12):1397-403
pubmed: 15482827
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111262
pubmed: 32806297
Endocr Rev. 2008 Jun;29(4):403-40
pubmed: 18451259
Mater Sci Eng C Mater Biol Appl. 2021 Feb;121:111839
pubmed: 33579477