The impact of Sambucus nigra L. extract on inflammation, oxidative stress and tissue remodeling in a rat model of lipopolysaccharide-induced subacute rhinosinusitis.
Animals
Anti-Inflammatory Agents
/ isolation & purification
Disease Models, Animal
Female
Fruit
Inflammation Mediators
/ antagonists & inhibitors
Lipopolysaccharides
/ toxicity
Oxidative Stress
/ drug effects
Plant Extracts
/ isolation & purification
Rats
Rats, Wistar
Rhinitis
/ chemically induced
Sambucus nigra
Sinusitis
/ chemically induced
Inflammation
Lipopolysaccharide
Oxidative stress
Rats
Sambucus nigra L.
Subacute rhinosinusitis
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
05
01
2021
accepted:
22
03
2021
pubmed:
22
4
2021
medline:
15
12
2021
entrez:
21
4
2021
Statut:
ppublish
Résumé
Rhinosinusitis is a common disorder related to inflammation of paranasal sinuses and nasal cavity mucosa. Herbal medicines could be an option in the treatment of rhinosinusitis due to their anti-inflammatory and anti-oxidative properties. The study aims to investigate the effect of intranasal Sambucus nigra L. subsp. nigra (SN) extract against inflammation, oxidative stress, and tissue remodeling in nasal and sinus mucosa, but also in serum, lungs, and brain, in Wistar rat model of subacute sinonasal inflammation induced by local administration of lipopolysaccharides (LPS), from Escherichia Coli. The cytokines (TNF-α, IL-1β, IL-6) and oxidative stress (malondialdehyde) in nasal mucosa, blood, lungs, and brain were analyzed. In addition, a histopathological examination was performed, and NF-kB, MMP2, MMP9, TIMP1 expressions were also evaluated in nasal mucosa. Both doses of LPS increased the production of cytokines in all the investigated tissues, especially in the nasal mucosa and blood (p < 0.01 and p < 0.05), and stimulated their secretion in the lungs, and partially in the brain. Malondialdehyde increased in all the investigated tissues (p < 0.01 and p < 0.05). In parallel, upregulation of NF-kB and MMP2 expressions with downregulation of TIMP1, particularly at high dose of LPS, was observed. SN extract reduced the local inflammatory response, maintained low levels of IL-6, TNF-α, and IL-1β. In lungs, SN reduced all cytokines levels while in the brain, the protective effect was noticed only on IL-6. Additionally, SN diminished lipid peroxidation and downregulated NF-kB in animals exposed to a low dose of LPS, with increased TIMP1 expression, while in animals treated with a high dose of LPS, SN increased NF-kB, MMP2, and MMP9 levels. In conclusion, SN extract diminished the inflammatory response, reduced generation of reactive oxygen species (ROS) and, influenced MMPs expressions, suggesting the benficial effect of SN extract on tissue remodeling in subacute rhinosinusitis and on systemic inflammatory response.
Identifiants
pubmed: 33881685
doi: 10.1007/s10787-021-00805-y
pii: 10.1007/s10787-021-00805-y
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Inflammation Mediators
0
Lipopolysaccharides
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
753-769Références
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