Single intracerebroventricular progranulin injection adversely affects the blood-brain barrier in experimental traumatic brain injury.
Animals
Animals, Newborn
Astrocytes
/ pathology
Behavior, Animal
/ drug effects
Blood-Brain Barrier
/ drug effects
Brain Injuries, Traumatic
/ pathology
Encephalitis
/ pathology
Injections, Intraventricular
Male
Mice
Mice, Inbred C57BL
Microglia
/ pathology
Primary Cell Culture
Progranulins
/ administration & dosage
Recombinant Proteins
/ administration & dosage
Tight Junction Proteins
/ biosynthesis
blood-brain barrier
neuroinflammation
neuroprotection
progranulin
traumatic brain injury
Journal
Journal of neurochemistry
ISSN: 1471-4159
Titre abrégé: J Neurochem
Pays: England
ID NLM: 2985190R
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
17
04
2021
received:
13
02
2021
accepted:
18
04
2021
pubmed:
27
4
2021
medline:
16
11
2021
entrez:
26
4
2021
Statut:
ppublish
Résumé
Progranulin (PGRN) is a neurotrophic and anti-inflammatory factor with protective effects in animal models of ischemic stroke, subarachnoid hemorrhage, and traumatic brain injury (TBI). Administration of recombinant (r) PGRN prevents exaggerated brain pathology after TBI in Grn-deficient mice, suggesting that local injection of recombinant progranulin (rPGRN) provides therapeutic benefit in the acute phase of TBI. To test this hypothesis, we subjected adult male C57Bl/6N mice to the controlled cortical impact model of TBI, administered a single dose of rPGRN intracerebroventricularly (ICV) shortly before the injury, and examined behavioral and biological effects up to 5 days post injury (dpi). The anti-inflammatory bioactivity of rPGRN was confirmed by its capability to inhibit the inflammation-induced hypertrophy of murine primary microglia and astrocytes in vitro. In C57Bl/6N mice, however, ICV administration of rPGRN failed to attenuate behavioral deficits over the 5-day observation period. (Immuno)histological gene and protein expression analyses at 5 dpi did not reveal a therapeutic benefit in terms of brain injury size, brain inflammation, glia activation, cell numbers in neurogenic niches, and neuronal damage. Instead, we observed a failure of TBI-induced mRNA upregulation of the tight junction protein occludin and increased extravasation of serum immunoglobulin G into the brain parenchyma at 5 dpi. In conclusion, single ICV administration of rPGRN had not the expected protective effects in the acute phase of murine TBI, but appeared to cause an aggravation of blood-brain barrier disruption. The data raise questions about putative PGRN-boosting approaches in other types of brain injuries and disease.
Substances chimiques
GRN protein, human
0
Progranulins
0
Recombinant Proteins
0
Tight Junction Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
342-357Informations de copyright
© 2021 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry.
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