Dilated Cardiomyopathy Mutations and Phosphorylation disrupt the Active Orientation of Cardiac Troponin C.
Calcium
/ metabolism
Cardiomyopathy, Dilated
/ genetics
Humans
Magnetic Resonance Spectroscopy
Models, Molecular
Multiprotein Complexes
/ chemistry
Mutation
Myocardium
/ metabolism
Phosphorylation
Protein Binding
Protein Domains
Tropomyosin
/ chemistry
Troponin C
/ chemistry
Troponin I
/ chemistry
Troponin T
/ chemistry
heritable cardiomyopathy
intrinsically disordered region
nuclear magnetic resonance spectroscopy
protein domain orientation
systolic heart failure
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
25 06 2021
25 06 2021
Historique:
received:
18
02
2021
revised:
07
04
2021
accepted:
20
04
2021
pubmed:
27
4
2021
medline:
31
8
2021
entrez:
26
4
2021
Statut:
ppublish
Résumé
Cardiac troponin (cTn) is made up of three subunits, cTnC, cTnI, and cTnT. The regulatory N-terminal domain of cTnC (cNTnC) controls cardiac muscle contraction in a calcium-dependent manner. We show that calcium-saturated cNTnC can adopt two different orientations, with the "active" orientation consistent with the 2020 cryo-EM structure of the activated cardiac thin filament by Yamada et al. Using solution NMR
Identifiants
pubmed: 33901537
pii: S0022-2836(21)00211-4
doi: 10.1016/j.jmb.2021.167010
pii:
doi:
Substances chimiques
Multiprotein Complexes
0
Tropomyosin
0
Troponin C
0
Troponin I
0
Troponin T
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
167010Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.