Intrauterine hCG application increases expression of endothelial cell-cell adhesion molecules in human.
ART
Embryo implantation
HCG
Mesenchymal stroma cells
Reproduction
Vascularization
Window of implantation
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
28
09
2020
accepted:
12
03
2021
pubmed:
28
4
2021
medline:
26
11
2021
entrez:
27
4
2021
Statut:
ppublish
Résumé
Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART.
Identifiants
pubmed: 33903941
doi: 10.1007/s00404-021-06031-9
pii: 10.1007/s00404-021-06031-9
pmc: PMC8553705
doi:
Substances chimiques
Cell Adhesion Molecules
0
Chorionic Gonadotropin
0
Types de publication
Journal Article
Meta-Analysis
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1587-1597Informations de copyright
© 2021. The Author(s).
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