Performance of different methods for testing polymyxin B: comparison of broth microdilution, agar dilution and MIC test strip in mcr-1 positive and negative Escherichia coli.
Enterobacterales
MIC test strip
agar dilution
antimicrobial susceptibility testing
broth microdilution
mobilized colistin resistance
polymyxin
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
25
03
2021
received:
09
08
2020
accepted:
13
04
2021
pubmed:
28
4
2021
medline:
6
8
2021
entrez:
27
4
2021
Statut:
ppublish
Résumé
Antimicrobial susceptibility testing with the last-resort antibiotics polymyxins (polymyxin B and colistin) is associated with several methodological issues. Currently, broth microdilution (BMD) is recommended for colistin and polymyxin B. BMD is laborious and the utility of alternative methods needs to be evaluated for polymyxin B susceptibility testing. In this study, using BMD as a reference method, the performance of agar dilution (AD) and MIC test strips (MTS) were evaluated in polymyxin B susceptibility testing. BMD, AD and MTS were used to determine MICs of 193 clinical isolates of Escherichia coli. Seventy-nine were positive for the polymyxin resistance gene mcr-1. Method performances were evaluated based on pair-wise agreements with the reference method (BMD) and statistical testing. AD and MTS showed an unacceptable number of very major errors (VMEs) compared with BMD, 9·3 and 10·7%, respectively. The essential agreement (EA) was low for AD (49·7%), but high for MTS (97·8%). However, statistical testing showed that MTS tended to yield a one-step lower MIC (P < 0·01) compared with BMD. The discordances observed with MTS and AD in comparison with BMD for polymyxin B susceptibility testing for E. coli suggest their inapplicability in routine testing. A large number of isolates clustered around the susceptibility breakpoint (2-4 mg l
Substances chimiques
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
MCR-1 protein, E coli
0
Polymyxin B
J2VZ07J96K
Colistin
Z67X93HJG1
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-205Subventions
Organisme : the Swedish Research Council Formas
ID : 2016-00640
Organisme : ALF-grants, Region Östergötland
ID : LIO-899871
Organisme : the Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
ID : CH2016-6707
Organisme : the Key Research and Development Program of Zhejiang Province
ID : 2015C03032
Informations de copyright
© 2021 The Society for Applied Microbiology.
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