Tension promotes kinetochore-microtubule release by Aurora B kinase.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 06 2021
07 06 2021
Historique:
received:
06
07
2020
revised:
06
02
2021
accepted:
24
03
2021
entrez:
27
4
2021
pubmed:
28
4
2021
medline:
12
10
2021
Statut:
ppublish
Résumé
To ensure accurate chromosome segregation, interactions between kinetochores and microtubules are regulated by a combination of mechanics and biochemistry. Tension provides a signal to discriminate attachment errors from bi-oriented kinetochores with sisters correctly attached to opposite spindle poles. Biochemically, Aurora B kinase phosphorylates kinetochores to destabilize interactions with microtubules. To link mechanics and biochemistry, current models regard tension as an input signal to locally regulate Aurora B activity. Here, we show that the outcome of kinetochore phosphorylation depends on tension. Using optogenetics to manipulate Aurora B at individual kinetochores, we find that kinase activity promotes microtubule release when tension is high. Conversely, when tension is low, Aurora B activity promotes depolymerization of kinetochore-microtubules while maintaining attachment. Thus, phosphorylation converts a catch-bond, in which tension stabilizes attachments, to a slip-bond, which releases microtubules under tension. We propose that tension is a signal inducing distinct error-correction pathways, with release or depolymerization being advantageous for typical errors characterized by high or low tension, respectively.
Identifiants
pubmed: 33904910
pii: 212027
doi: 10.1083/jcb.202007030
pmc: PMC8082439
pii:
doi:
Substances chimiques
TNS1 protein, human
0
Tensins
0
AURKB protein, human
EC 2.7.11.1
Aurora Kinase B
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM118510
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122475
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130298
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA193417
Pays : United States
Informations de copyright
© 2021 Chen et al.
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